SYSTEMS AND METHODS ENABLING PATCH-CLAMP RE-USE

A method for cleaning patch-clamp glass pipette electrodes that enables their re-use. By immersing pipette tips or planar patch clamp chips into a detergent, followed by rinsing, pipettes and planar patch clamp chips were re-usable at least ten times with little to no degradation in signal fidelity,...

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Hauptverfasser: FOREST, Craig R, STOY, William, KOLB, Ilya, ROUSSEAU, Erin
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Sprache:eng ; fre ; ger
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creator FOREST, Craig R
STOY, William
KOLB, Ilya
ROUSSEAU, Erin
description A method for cleaning patch-clamp glass pipette electrodes that enables their re-use. By immersing pipette tips or planar patch clamp chips into a detergent, followed by rinsing, pipettes and planar patch clamp chips were re-usable at least ten times with little to no degradation in signal fidelity, in experimental preparations ranging from human embryonic kidney cells to neurons in culture, slices, and in vivo.
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subjects CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
PHYSICS
TESTING
TRANSPORTING
title SYSTEMS AND METHODS ENABLING PATCH-CLAMP RE-USE
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