PRODUCTION METHOD FOR OLIGONUCLEOTIDES

PRODUCTION METHOD FOR OLIGONUCLEOTIDES

A method for producing an oligonucleotide that can complete a condensation reaction to improve a condensation yield, and can markedly reduce impurities such as single base deletion form (N-1mer) and the like is provided. A method for producing an oligonucleotide, including a step of removing a tempo...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: ICHIMARU, Taisuke, TAKAHASHI, Daisuke, HIRAI, Kunihiro
Format: Patent
Sprache:eng ; fre ; ger
Schlagworte:
CHEMISTRY
DERIVATIVES THEREOF
METALLURGY
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC CHEMISTRY
SUGARS
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:A method for producing an oligonucleotide that can complete a condensation reaction to improve a condensation yield, and can markedly reduce impurities such as single base deletion form (N-1mer) and the like is provided. A method for producing an oligonucleotide, including a step of removing a temporary protecting group of a 5'-hydroxyl group by adding an acid to a protected oligonucleotide having a phosphorothioate (PS), phosphate (PO) or H-phosphonate moiety, and deacylating a by-product having an acylated 5'-hydroxyl group by solvolysis.