GENETIC CONSTRUCT AND USES THEREOF

A spore-forming cell comprising a first nucleotide sequence encoding a riboswitch and a transcriptional activator, wherein the riboswitch modulates translation of the transcriptional activator in response to a First inducer, and a second nucleotide sequence encoding a target gene that regulates spor...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: ZYGOUROPOULOU, Maria, BLASCO, Ines Canadas, MINTON, Nigel, GROOTHUIS, Daphne
Format: Patent
Sprache:eng ; fre ; ger
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Beschreibung
Zusammenfassung:A spore-forming cell comprising a first nucleotide sequence encoding a riboswitch and a transcriptional activator, wherein the riboswitch modulates translation of the transcriptional activator in response to a First inducer, and a second nucleotide sequence encoding a target gene that regulates spore formation, wherein the target gene is expressed in response to a second inducer and translation of the transcriptional activator. Also provided is a method of culturing a spore-forming cell and a method of creating a spore, as well as uses of spore-forming cells and spores.