DIRECT DETECTION OF SINGLE MOLECULES ON MICROPARTICLES

DIRECT DETECTION OF SINGLE MOLECULES ON MICROPARTICLES

The disclosure provides methods of analyzing an analyte of interest in a biological sample using fluorescent agents and macroconjugates which comprise a core containing a crosslinked polymer or protein, tags, specific binding members or fragments thereof, and optionally carrier proteins. Also provid...

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Bibliographische Detailangaben
Hauptverfasser: PROSTKO, John, HAACK, Richard, TETIN, Sergey Y, POPE, Mark R, MACDONALD, Patrick J, QIU, Xiaoxing, RUAN, Qiaoqiao, SWIFT, Kerry M, CALFIN, Brenda, LIN, Zhen, BOGDAN, M. Felicia
Format: Patent
Sprache:eng ; fre ; ger
Schlagworte:
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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Beschreibung
Zusammenfassung:The disclosure provides methods of analyzing an analyte of interest in a biological sample using fluorescent agents and macroconjugates which comprise a core containing a crosslinked polymer or protein, tags, specific binding members or fragments thereof, and optionally carrier proteins. Also provided are methods of analyzing two or more analytes of interest in a biological sample in a single assay using microparticles and detection conjugates comprising different fluorophore labels, acquiring transmitted light and fluorescent images of the microparticles, and using a customized image analysis process to analyze the acquired images.