COMPOSITIONS AND METHODS COMPRISING SUBTILISIN VARIANTS
The present invention provides isolated subtilisin variants. Specifically, a subtilisin variant is a mature form having proteolytic activity and comprises an amino acid sequence comprising the combination of amino acid substitutions: T022R, S101G, S103A, V104I, A232V, and Q245R, wherein the variant...
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| creator | SOUTER, Philip Frank CASCAO-PEREIRA, Luis Gustavo POULOSE, Ayrookaran, J YAO, Jian ESTELL, David, A COLLIER, Katherine, D AMIN, Neelam, S AUGUSTYN, Katherine ALEKSEYEV, Viktor Yuryevich KELLIS JR., James, T WARD, Glenn Steven |
| description | The present invention provides isolated subtilisin variants. Specifically, a subtilisin variant is a mature form having proteolytic activity and comprises an amino acid sequence comprising the combination of amino acid substitutions: T022R, S101G, S103A, V104I, A232V, and Q245R, wherein the variant is a variant of Bacillus lentus subtilisin GG36 protease comprising the amino acid sequence shown in SEQ ID NO:2, and wherein the variant has at least 90% amino acid sequence identity to the amino acid sequence of SEQ ID NO: 2, and wherein the amino acid positions of the subtilisin variant are numbered according to the numbering of corresponding amino acid positions in the amino acid sequence of B. amyloliquefaciens subtilisin BPN' set forth as SEQ ID NO: 1. In addition, the present invention provides compositions comprising these subtilisin variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these subtilisin variants and methods of cleaning using the cleaning compositions. |
| format | Patent |
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Specifically, a subtilisin variant is a mature form having proteolytic activity and comprises an amino acid sequence comprising the combination of amino acid substitutions: T022R, S101G, S103A, V104I, A232V, and Q245R, wherein the variant is a variant of Bacillus lentus subtilisin GG36 protease comprising the amino acid sequence shown in SEQ ID NO:2, and wherein the variant has at least 90% amino acid sequence identity to the amino acid sequence of SEQ ID NO: 2, and wherein the amino acid positions of the subtilisin variant are numbered according to the numbering of corresponding amino acid positions in the amino acid sequence of B. amyloliquefaciens subtilisin BPN' set forth as SEQ ID NO: 1. In addition, the present invention provides compositions comprising these subtilisin variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these subtilisin variants and methods of cleaning using the cleaning compositions.</description><language>eng ; fre ; ger</language><subject>ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES ; BEER ; BIOCHEMISTRY ; CANDLES ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; DETERGENT COMPOSITIONS ; DETERGENTS ; ENZYMOLOGY ; FATTY ACIDS THEREFROM ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; RECOVERY OF GLYCEROL ; RESIN SOAPS ; SOAP OR SOAP-MAKING ; SPIRITS ; USE OF SINGLE SUBSTANCES AS DETERGENTS ; VINEGAR ; WINE</subject><creationdate>2021</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210825&DB=EPODOC&CC=EP&NR=3868881A2$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210825&DB=EPODOC&CC=EP&NR=3868881A2$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>SOUTER, Philip Frank</creatorcontrib><creatorcontrib>CASCAO-PEREIRA, Luis Gustavo</creatorcontrib><creatorcontrib>POULOSE, Ayrookaran, J</creatorcontrib><creatorcontrib>YAO, Jian</creatorcontrib><creatorcontrib>ESTELL, David, A</creatorcontrib><creatorcontrib>COLLIER, Katherine, D</creatorcontrib><creatorcontrib>AMIN, Neelam, S</creatorcontrib><creatorcontrib>AUGUSTYN, Katherine</creatorcontrib><creatorcontrib>ALEKSEYEV, Viktor Yuryevich</creatorcontrib><creatorcontrib>KELLIS JR., James, T</creatorcontrib><creatorcontrib>WARD, Glenn Steven</creatorcontrib><title>COMPOSITIONS AND METHODS COMPRISING SUBTILISIN VARIANTS</title><description>The present invention provides isolated subtilisin variants. Specifically, a subtilisin variant is a mature form having proteolytic activity and comprises an amino acid sequence comprising the combination of amino acid substitutions: T022R, S101G, S103A, V104I, A232V, and Q245R, wherein the variant is a variant of Bacillus lentus subtilisin GG36 protease comprising the amino acid sequence shown in SEQ ID NO:2, and wherein the variant has at least 90% amino acid sequence identity to the amino acid sequence of SEQ ID NO: 2, and wherein the amino acid positions of the subtilisin variant are numbered according to the numbering of corresponding amino acid positions in the amino acid sequence of B. amyloliquefaciens subtilisin BPN' set forth as SEQ ID NO: 1. In addition, the present invention provides compositions comprising these subtilisin variants. 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Specifically, a subtilisin variant is a mature form having proteolytic activity and comprises an amino acid sequence comprising the combination of amino acid substitutions: T022R, S101G, S103A, V104I, A232V, and Q245R, wherein the variant is a variant of Bacillus lentus subtilisin GG36 protease comprising the amino acid sequence shown in SEQ ID NO:2, and wherein the variant has at least 90% amino acid sequence identity to the amino acid sequence of SEQ ID NO: 2, and wherein the amino acid positions of the subtilisin variant are numbered according to the numbering of corresponding amino acid positions in the amino acid sequence of B. amyloliquefaciens subtilisin BPN' set forth as SEQ ID NO: 1. In addition, the present invention provides compositions comprising these subtilisin variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these subtilisin variants and methods of cleaning using the cleaning compositions.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES BEER BIOCHEMISTRY CANDLES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DETERGENT COMPOSITIONS DETERGENTS ENZYMOLOGY FATTY ACIDS THEREFROM METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS RECOVERY OF GLYCEROL RESIN SOAPS SOAP OR SOAP-MAKING SPIRITS USE OF SINGLE SUBSTANCES AS DETERGENTS VINEGAR WINE |
| title | COMPOSITIONS AND METHODS COMPRISING SUBTILISIN VARIANTS |
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