PRETREATMENT METHOD FOR FLUORESCENT IMAGE DIAGNOSIS

The purpose of the present invention is to provide a method for allowing a target cell to emit light, in which the target cell is present inside of a cell mass and is aimed to emit light by a fluorescent probe. The present invention provides a method for allowing the target cell to emit fluorescence...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	TEZUKA, Yoshiaki, HIRAWAKE, Kazumasa, MINOURA, Itsushi
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The purpose of the present invention is to provide a method for allowing a target cell to emit light, in which the target cell is present inside of a cell mass and is aimed to emit light by a fluorescent probe. The present invention provides a method for allowing the target cell to emit fluorescence comprising removing calcium ions from within the cell mass or a tissue containing the target cell and contacting the cell mass or the tissue with the fluorescent probe which emits fluorescence when contacted with or taken up into the target cell, thereby allowing the target cell present inside of the cell mass or the tissue to emit light, and thus can be used in a detection of the target cell in a sample, such as a clinical sample, in which the target cell may not be present on the surface of the sample.