METHOD FOR PRODUCING HAIRPIN SINGLE-STRANDED RNA MOLECULE

The present invention provides a method for producing a hairpin single-stranded RNA molecule capable of inhibiting expression of a target gene, the method comprising: (i) an annealing step of annealing a first single-stranded oligoRNA molecule and a second single-stranded oligoRNA molecule; and (ii)...

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Bibliographische Detailangaben
Hauptverfasser:	TAKASHINA, Ayumi, INADA, Hideaki, OKIMURA, Keiichi, ISEKI, Katsuhiko, SANOSAKA, Masato
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention provides a method for producing a hairpin single-stranded RNA molecule capable of inhibiting expression of a target gene, the method comprising: (i) an annealing step of annealing a first single-stranded oligoRNA molecule and a second single-stranded oligoRNA molecule; and (ii) a ligation step of ligating 3' end of the first single-stranded oligoRNA molecule and 5' end of the second single-stranded oligoRNA molecule by an Rnl2 family ligase, wherein a sequence produced by ligating the first single-stranded oligoRNA molecule and the second single-stranded oligoRNA molecule comprises a gene expression-inhibiting sequence for the target gene.