IMPROVING DETECTION OF MICROORGANISMS

The present invention discloses method and means for rapid detection and quantification of specific live and recovered microorganisms in a sample, comprising steps of contacting the sample with a detection cocktail, the detection cocktail comprises: (i) a nutrient medium comprising at least one of cell growth stimulators from potential-host of the pathogen for accelerated selective growing and multiplying of said microorganism; (ii) at least one fluorescent marker molecule for detection of intracellular metabolism by a sensor, (iii) at least one metabolism activator for specifically increasing intracellular metabolism of said specific microorganism and increasing intracellular concentration of said fluorescent marker molecule into said specific microorganism; (iv) determination of numerical range for the level of intracellular fluorescence intensity of the said marker for the differentiation of specific recovered microorganisms from all other objects and (v) determining recovered specific microbial cells by measurement of intra-cellular concentration of metabolism markers in each microscope visualized cell. The rapid detection and quantification of specific live and recovered microorganisms in a sample is further performed by: (i) measuring of fluorescent intensity of the marker molecules in individual cells by the sensor, while intensity levels correlated to predetermined metabolism level of said specific microorganism; (ii) determining the gray level to a predetermined threshold of metabolism; and (iii) further correlating the gray levels above the predetermined threshold with quantity of recovered high metabolic-active said microorganism in the sample.