THREE-CHANNEL FLUORESCENCE POSITIONING SUPER-RESOLUTION BIOLOGICAL MICROSCOPE SYSTEM AND METHOD
A three-channel fluorescence localization super-resolution biological microscope system includes a microscope main body, an illumination module (2), an imaging module (3) and a three-dimensional nanoscale sample locking module (4). The illumination module (2) is used to realize three channels, wide-...
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creator | WANG, Luning YU, Lujia WANG, Ying XU, Daiying DU, Shengwang QI, Wen |
description | A three-channel fluorescence localization super-resolution biological microscope system includes a microscope main body, an illumination module (2), an imaging module (3) and a three-dimensional nanoscale sample locking module (4). The illumination module (2) is used to realize three channels, wide-field and semi-total internal reflection illumination and total internal reflection illumination and is adjustable in an illumination mode. Illumination light generated by the illumination module (2) illuminates a sample through an objective lens. Fluorescent light emitted from the sample is allocated by a light splitter (8) of a fixed ratio after passing through a first multicolor reflector (6) and a front imaging lens (7), one part is allocated into the three-dimensional nanoscale sample locking module (4), and the remaining part is allocated into the imaging module. A three-channel fluorescence localization super-resolution biological microscope method is disclosed. |
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subjects | INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS OPTICS PHYSICS TESTING |
title | THREE-CHANNEL FLUORESCENCE POSITIONING SUPER-RESOLUTION BIOLOGICAL MICROSCOPE SYSTEM AND METHOD |
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