METHODS FOR SEAMLESS NUCLEIC ACID ASSEMBLY

METHODS FOR SEAMLESS NUCLEIC ACID ASSEMBLY

Provided herein are methods, systems, and compositions for seamless nucleic acid assembly. Methods, systems, and compositions as provided herein provide for efficient assembly of nucleic acids without primer removal. Methods, systems, and compositions for seamless nucleic acid assembly comprise use...

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Hauptverfasser: NUGENT, Rebecca, RAYNARD, Nathan, CHEN, Siyuan, LEE, Elian
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Sprache:eng ; fre ; ger
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BEER
BIOCHEMISTRY
CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
THEIR RELEVANT APPARATUS
TRANSPORTING
VINEGAR
WINE
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creator NUGENT, Rebecca
RAYNARD, Nathan
CHEN, Siyuan
LEE, Elian
description Provided herein are methods, systems, and compositions for seamless nucleic acid assembly. Methods, systems, and compositions as provided herein provide for efficient assembly of nucleic acids without primer removal. Methods, systems, and compositions for seamless nucleic acid assembly comprise use of an endonuclease or exonuclease, optionally in conjunction with additional enzymes to assemble nucleic acids or polynucleotides.
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subjects BEER
BIOCHEMISTRY
CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
THEIR RELEVANT APPARATUS
TRANSPORTING
VINEGAR
WINE
title METHODS FOR SEAMLESS NUCLEIC ACID ASSEMBLY
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