SUBTILISIN VARIANTS HAVING A MUTATION IN THE S2 OR S2' POCKET
The invention relates to a subtilisin BPN' variant or homologue thereof, comprising the following mutations compared to subtilisin BPN' represented by SEQUENCE ID NO: 2 or a homologue sequence thereof: - a deletion of the amino acids corresponding to positions 75-83; - a mutation at the am...
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| creator | NUIJENS, Timo TOPLAK, Ana QUAEDFLIEG, Peter Jan Leonard Mario |
| description | The invention relates to a subtilisin BPN' variant or homologue thereof, comprising the following mutations compared to subtilisin BPN' represented by SEQUENCE ID NO: 2 or a homologue sequence thereof:
- a deletion of the amino acids corresponding to positions 75-83;
- a mutation at the amino acid position corresponding to S221, the mutation corresponding to S221C or S221selenocysteine, preferably S221C;
- at least one further mutation selected from the group consisting of amino acid positions corresponding to F189W, F189Y, S33D, S33T, N218D, N218T, N218E, N62D, N62S, N62W, and N62Y; preferably a mutation at the amino acid position corresponding to P225;
wherein the amino acid positions are defined according to the sequence of subtilisin BPN' represented by SEQUENCE ID NO: 2. The invention further relates to enzymatically synthesizing a peptide. |
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- a deletion of the amino acids corresponding to positions 75-83;
- a mutation at the amino acid position corresponding to S221, the mutation corresponding to S221C or S221selenocysteine, preferably S221C;
- at least one further mutation selected from the group consisting of amino acid positions corresponding to F189W, F189Y, S33D, S33T, N218D, N218T, N218E, N62D, N62S, N62W, and N62Y; preferably a mutation at the amino acid position corresponding to P225;
wherein the amino acid positions are defined according to the sequence of subtilisin BPN' represented by SEQUENCE ID NO: 2. The invention further relates to enzymatically synthesizing a peptide.</description><language>eng ; fre ; ger</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2020</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20200401&DB=EPODOC&CC=EP&NR=3628046A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25563,76318</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20200401&DB=EPODOC&CC=EP&NR=3628046A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>NUIJENS, Timo</creatorcontrib><creatorcontrib>TOPLAK, Ana</creatorcontrib><creatorcontrib>QUAEDFLIEG, Peter Jan Leonard Mario</creatorcontrib><title>SUBTILISIN VARIANTS HAVING A MUTATION IN THE S2 OR S2' POCKET</title><description>The invention relates to a subtilisin BPN' variant or homologue thereof, comprising the following mutations compared to subtilisin BPN' represented by SEQUENCE ID NO: 2 or a homologue sequence thereof:
- a deletion of the amino acids corresponding to positions 75-83;
- a mutation at the amino acid position corresponding to S221, the mutation corresponding to S221C or S221selenocysteine, preferably S221C;
- at least one further mutation selected from the group consisting of amino acid positions corresponding to F189W, F189Y, S33D, S33T, N218D, N218T, N218E, N62D, N62S, N62W, and N62Y; preferably a mutation at the amino acid position corresponding to P225;
wherein the amino acid positions are defined according to the sequence of subtilisin BPN' represented by SEQUENCE ID NO: 2. The invention further relates to enzymatically synthesizing a peptide.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2020</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZLANDnUK8fTxDPb0UwhzDPJ09AsJVvBwDPP0c1dwVPANDXEM8fT3UwDKhni4KgQbKfgHAUl1hQB_Z2_XEB4G1rTEnOJUXijNzaDg5hri7KGbWpAfn1pckJicmpdaEu8aYGxmZGFgYuZoaEyEEgC0tCjz</recordid><startdate>20200401</startdate><enddate>20200401</enddate><creator>NUIJENS, Timo</creator><creator>TOPLAK, Ana</creator><creator>QUAEDFLIEG, Peter Jan Leonard Mario</creator><scope>EVB</scope></search><sort><creationdate>20200401</creationdate><title>SUBTILISIN VARIANTS HAVING A MUTATION IN THE S2 OR S2' POCKET</title><author>NUIJENS, Timo ; TOPLAK, Ana ; QUAEDFLIEG, Peter Jan Leonard Mario</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_EP3628046A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre ; ger</language><creationdate>2020</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>NUIJENS, Timo</creatorcontrib><creatorcontrib>TOPLAK, Ana</creatorcontrib><creatorcontrib>QUAEDFLIEG, Peter Jan Leonard Mario</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>NUIJENS, Timo</au><au>TOPLAK, Ana</au><au>QUAEDFLIEG, Peter Jan Leonard Mario</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>SUBTILISIN VARIANTS HAVING A MUTATION IN THE S2 OR S2' POCKET</title><date>2020-04-01</date><risdate>2020</risdate><abstract>The invention relates to a subtilisin BPN' variant or homologue thereof, comprising the following mutations compared to subtilisin BPN' represented by SEQUENCE ID NO: 2 or a homologue sequence thereof:
- a deletion of the amino acids corresponding to positions 75-83;
- a mutation at the amino acid position corresponding to S221, the mutation corresponding to S221C or S221selenocysteine, preferably S221C;
- at least one further mutation selected from the group consisting of amino acid positions corresponding to F189W, F189Y, S33D, S33T, N218D, N218T, N218E, N62D, N62S, N62W, and N62Y; preferably a mutation at the amino acid position corresponding to P225;
wherein the amino acid positions are defined according to the sequence of subtilisin BPN' represented by SEQUENCE ID NO: 2. The invention further relates to enzymatically synthesizing a peptide.</abstract><oa>free_for_read</oa></addata></record> |
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| language | eng ; fre ; ger |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | SUBTILISIN VARIANTS HAVING A MUTATION IN THE S2 OR S2' POCKET |
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