METHOD FOR PRODUCING DOUBLE STRANDED POLYNUCLEOTIDES BASED ON OLIGONUCLEOTIDES WITH SELECTED AND DIFFERENT MELTING TEMPERATURES

METHOD FOR PRODUCING DOUBLE STRANDED POLYNUCLEOTIDES BASED ON OLIGONUCLEOTIDES WITH SELECTED AND DIFFERENT MELTING TEMPERATURES

A method for identifying a group of single-stranded oligonucleotides for self-assembly into a double-stranded polynucleotide, the group comprising a plurality of overlapping complementary oligonucleotides, wherein each overlap between complementary oligonucleotides is selected to have a melting temp...

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Hauptverfasser: SANCHES-KUIPER, Raquel, ADITYA, Sangeeta, BRENNAN, Joseph, BYGRAVE, Daniel
Format: Patent
Sprache:eng ; fre ; ger
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:A method for identifying a group of single-stranded oligonucleotides for self-assembly into a double-stranded polynucleotide, the group comprising a plurality of overlapping complementary oligonucleotides, wherein each overlap between complementary oligonucleotides is selected to have a melting temperature (T m ) that differs from the melting temperatures of all other overlapping complementary oligonucleotides in the group.