NUCLEIC ACID MUTATION DETECTION USING MAGNETIC BEAD ACTUATION AND DETECTION
The present invention relates to a method for distinguishing mutations, single nucleotide polymorphisms or other variants in a target nucleic acid sequence from the wild-type sequence in a sample. The method comprises the steps of surface binding magnetic beads via a sandwich hybridization in which...
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| creator | HIELTJES, Martinus Johannes FEITSMA, Harma Martine EVERS, Toon Hendrik DE REGT, Bram VAN ZON, Joannes Baptist Adrianus Dionisius |
| description | The present invention relates to a method for distinguishing mutations, single nucleotide polymorphisms or other variants in a target nucleic acid sequence from the wild-type sequence in a sample. The method comprises the steps of surface binding magnetic beads via a sandwich hybridization in which a bead-bound probe hybridizes with one end of a target nucleic acid, and a surface-bound probe hybridizes with the other end of the same target nucleic acid, applying stringency on the hybridization by magnetic force and/or temperature, determining the amount of magnetic beads remaining attached to the surface, and correlating the amount of magnetic beads remaining attached to the surface with the presence and/or absence of mutations, SNP variants or other variants in the target nucleic acid. |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | NUCLEIC ACID MUTATION DETECTION USING MAGNETIC BEAD ACTUATION AND DETECTION |
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