APPARATUS FOR SINGLE MOLECULAR SEQUENCING AND METHOD OF SEQUENCING NUCLEIC ACID MOLECULES
An apparatus (100, 100a) suitable for single molecule sequencing. The apparatus (100, 100a) includes at least one nanowell (110), a plurality of nucleic acid immobilization moieties (120a, 120b, 120c, 120d, 120e), and a plurality of types of nucleic acid fragments (130a, 130b, 130c, 130d, 130e). The...
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| creator | PAN, Chao-Chi CIOU, Jian-Hao CHIOU, Chung-Fan TSAI, Ching-Wei CHEN, Bor-Huah |
| description | An apparatus (100, 100a) suitable for single molecule sequencing. The apparatus (100, 100a) includes at least one nanowell (110), a plurality of nucleic acid immobilization moieties (120a, 120b, 120c, 120d, 120e), and a plurality of types of nucleic acid fragments (130a, 130b, 130c, 130d, 130e). The nanowell (110) has an observation zone (112). The nucleic acid immobilization moieties (120a, 120b, 120c, 120d, 120e) are disposed in or proximate to the observation zone (112). The nucleic acid fragments (130a, 130b, 130c, 130d, 130e) are immobilized to the nucleic acid immobilization moieties (120a, 120b, 120c, 120d, 120e), respectively. At least one polymerase (140a, 140b, 140c, 140d) is disposed in the observation zone (112). A method of sequencing nucleic acid molecules using the above-mentioned apparatus is provided. |
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The apparatus (100, 100a) includes at least one nanowell (110), a plurality of nucleic acid immobilization moieties (120a, 120b, 120c, 120d, 120e), and a plurality of types of nucleic acid fragments (130a, 130b, 130c, 130d, 130e). The nanowell (110) has an observation zone (112). The nucleic acid immobilization moieties (120a, 120b, 120c, 120d, 120e) are disposed in or proximate to the observation zone (112). The nucleic acid fragments (130a, 130b, 130c, 130d, 130e) are immobilized to the nucleic acid immobilization moieties (120a, 120b, 120c, 120d, 120e), respectively. At least one polymerase (140a, 140b, 140c, 140d) is disposed in the observation zone (112). A method of sequencing nucleic acid molecules using the above-mentioned apparatus is provided.</description><language>eng ; fre ; ger</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS ; OPTICS ; PHYSICS ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; TESTING ; VINEGAR ; WINE</subject><creationdate>2018</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20181219&DB=EPODOC&CC=EP&NR=3415642A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20181219&DB=EPODOC&CC=EP&NR=3415642A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>PAN, Chao-Chi</creatorcontrib><creatorcontrib>CIOU, Jian-Hao</creatorcontrib><creatorcontrib>CHIOU, Chung-Fan</creatorcontrib><creatorcontrib>TSAI, Ching-Wei</creatorcontrib><creatorcontrib>CHEN, Bor-Huah</creatorcontrib><title>APPARATUS FOR SINGLE MOLECULAR SEQUENCING AND METHOD OF SEQUENCING NUCLEIC ACID MOLECULES</title><description>An apparatus (100, 100a) suitable for single molecule sequencing. The apparatus (100, 100a) includes at least one nanowell (110), a plurality of nucleic acid immobilization moieties (120a, 120b, 120c, 120d, 120e), and a plurality of types of nucleic acid fragments (130a, 130b, 130c, 130d, 130e). The nanowell (110) has an observation zone (112). The nucleic acid immobilization moieties (120a, 120b, 120c, 120d, 120e) are disposed in or proximate to the observation zone (112). The nucleic acid fragments (130a, 130b, 130c, 130d, 130e) are immobilized to the nucleic acid immobilization moieties (120a, 120b, 120c, 120d, 120e), respectively. At least one polymerase (140a, 140b, 140c, 140d) is disposed in the observation zone (112). A method of sequencing nucleic acid molecules using the above-mentioned apparatus is provided.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>ENZYMOLOGY</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS</subject><subject>OPTICS</subject><subject>PHYSICS</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>SPIRITS</subject><subject>TESTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2018</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZIh0DAhwDHIMCQ1WcPMPUgj29HP3cVXw9fdxdQ71cQQKuAaGuvo5A4UVHP1cFHxdQzz8XRT83ZAl_EKdfVw9nRUcnT1dYFpdg3kYWNMSc4pTeaE0N4OCm2uIs4duakF-fGpxQWJyal5qSbxrgLGJoamZiZGjoTERSgC9QTEC</recordid><startdate>20181219</startdate><enddate>20181219</enddate><creator>PAN, Chao-Chi</creator><creator>CIOU, Jian-Hao</creator><creator>CHIOU, Chung-Fan</creator><creator>TSAI, Ching-Wei</creator><creator>CHEN, Bor-Huah</creator><scope>EVB</scope></search><sort><creationdate>20181219</creationdate><title>APPARATUS FOR SINGLE MOLECULAR SEQUENCING AND METHOD OF SEQUENCING NUCLEIC ACID MOLECULES</title><author>PAN, Chao-Chi ; CIOU, Jian-Hao ; CHIOU, Chung-Fan ; TSAI, Ching-Wei ; CHEN, Bor-Huah</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_EP3415642A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre ; ger</language><creationdate>2018</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>ENZYMOLOGY</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS</topic><topic>OPTICS</topic><topic>PHYSICS</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>SPIRITS</topic><topic>TESTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>PAN, Chao-Chi</creatorcontrib><creatorcontrib>CIOU, Jian-Hao</creatorcontrib><creatorcontrib>CHIOU, Chung-Fan</creatorcontrib><creatorcontrib>TSAI, Ching-Wei</creatorcontrib><creatorcontrib>CHEN, Bor-Huah</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>PAN, Chao-Chi</au><au>CIOU, Jian-Hao</au><au>CHIOU, Chung-Fan</au><au>TSAI, Ching-Wei</au><au>CHEN, Bor-Huah</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>APPARATUS FOR SINGLE MOLECULAR SEQUENCING AND METHOD OF SEQUENCING NUCLEIC ACID MOLECULES</title><date>2018-12-19</date><risdate>2018</risdate><abstract>An apparatus (100, 100a) suitable for single molecule sequencing. The apparatus (100, 100a) includes at least one nanowell (110), a plurality of nucleic acid immobilization moieties (120a, 120b, 120c, 120d, 120e), and a plurality of types of nucleic acid fragments (130a, 130b, 130c, 130d, 130e). The nanowell (110) has an observation zone (112). The nucleic acid immobilization moieties (120a, 120b, 120c, 120d, 120e) are disposed in or proximate to the observation zone (112). The nucleic acid fragments (130a, 130b, 130c, 130d, 130e) are immobilized to the nucleic acid immobilization moieties (120a, 120b, 120c, 120d, 120e), respectively. At least one polymerase (140a, 140b, 140c, 140d) is disposed in the observation zone (112). A method of sequencing nucleic acid molecules using the above-mentioned apparatus is provided.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS OPTICS PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE |
| title | APPARATUS FOR SINGLE MOLECULAR SEQUENCING AND METHOD OF SEQUENCING NUCLEIC ACID MOLECULES |
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