MULTIPLE AMPLIFICATION CYCLE DETECTION

Methods and devices are provided for simultaneously amplifying a plurality of sample wells for a predetermined amount of amplification, detecting whether amplification has occurred in a first set of the wells, amplifying for an additional amount of amplification and detecting whether amplification h...

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Hauptverfasser: RASMUSSEN, Randy P, EYRE, David J, CRISP, Robert John, ROBBINS, Thomas Charles, HEMMERT, Andrew Clinton, BARKER CAMPBELL, Elizabeth Diana
Format: Patent
Sprache:eng ; fre ; ger
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Zusammenfassung:Methods and devices are provided for simultaneously amplifying a plurality of sample wells for a predetermined amount of amplification, detecting whether amplification has occurred in a first set of the wells, amplifying for an additional amount of amplification and detecting whether amplification has occurred in a second set of the wells. Methods are also provided for analyzing a target nucleic acid sequence using melt curves that were generated in a plurality of amplification cycles.