PROCESS FOR THE MODIFICATION OF A GLYCOPROTEIN USING A GLYCOSYLTRANSFERASE THAT IS OR IS DERIVED FROM A BETA-(1,4)-N-ACETYLGALACTOSAMINYLTRANSFERASE
The present invention relates to a process for the enzymatic modification of a glycoprotein. The process comprises the step of contacting a glycoprotein comprising a glycan comprising a terminal GlcNAc-moiety, in the presence of glycosyltransferase that is, or is derived from, a ²-(1,4)-N-acetylgala...
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| creator | WIJDEVEN, Maria Antonia VAN DELFT, Floris Louis VAN BERKEL, Sander Sebastiaan VAN GEEL, Remon |
| description | The present invention relates to a process for the enzymatic modification of a glycoprotein. The process comprises the step of contacting a glycoprotein comprising a glycan comprising a terminal GlcNAc-moiety, in the presence of glycosyltransferase that is, or is derived from, a ²-(1,4)-N-acetylgalactosaminyltransferase, with a non-natural sugar-derivative nucleotide. The non-natural sugar-derivative nucleotide is according to formula (3):
wherein A is selected from the group consisting of -N 3 , -C(O)R 3 , -(CH 2 ) i C¡C-R 4 , -SH, -SC(O)R 8 , -SC(O)OR 8 , -SC(S)OR 8 , -F, -Cl, -Br -I, -OS(O) 2 R 5 , terminal C 2 - C 24 alkenyl groups, C 3 - C 5 cycloalkenyl groups, C 4 - C 8 alkadienyl groups, terminal C 3 - C 24 allenyl groups and amino groups. The invention further relates to a glycoprotein obtainable by the process according to the invention, to a bioconjugate that can be obtained by conjugating the glycoprotein with a linker-conjugate, and to ²-(1,4)-N-acetylgalactosaminyltransferases that can be used in preparing the the glycoprotein according to the invention. |
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wherein A is selected from the group consisting of -N 3 , -C(O)R 3 , -(CH 2 ) i C¡C-R 4 , -SH, -SC(O)R 8 , -SC(O)OR 8 , -SC(S)OR 8 , -F, -Cl, -Br -I, -OS(O) 2 R 5 , terminal C 2 - C 24 alkenyl groups, C 3 - C 5 cycloalkenyl groups, C 4 - C 8 alkadienyl groups, terminal C 3 - C 24 allenyl groups and amino groups. The invention further relates to a glycoprotein obtainable by the process according to the invention, to a bioconjugate that can be obtained by conjugating the glycoprotein with a linker-conjugate, and to ²-(1,4)-N-acetylgalactosaminyltransferases that can be used in preparing the the glycoprotein according to the invention.</description><language>eng ; fre ; ger</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; HUMAN NECESSITIES ; HYGIENE ; MEDICAL OR VETERINARY SCIENCE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2024</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20241030&DB=EPODOC&CC=EP&NR=3354726B1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20241030&DB=EPODOC&CC=EP&NR=3354726B1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>WIJDEVEN, Maria Antonia</creatorcontrib><creatorcontrib>VAN DELFT, Floris Louis</creatorcontrib><creatorcontrib>VAN BERKEL, Sander Sebastiaan</creatorcontrib><creatorcontrib>VAN GEEL, Remon</creatorcontrib><title>PROCESS FOR THE MODIFICATION OF A GLYCOPROTEIN USING A GLYCOSYLTRANSFERASE THAT IS OR IS DERIVED FROM A BETA-(1,4)-N-ACETYLGALACTOSAMINYLTRANSFERASE</title><description>The present invention relates to a process for the enzymatic modification of a glycoprotein. The process comprises the step of contacting a glycoprotein comprising a glycan comprising a terminal GlcNAc-moiety, in the presence of glycosyltransferase that is, or is derived from, a ²-(1,4)-N-acetylgalactosaminyltransferase, with a non-natural sugar-derivative nucleotide. The non-natural sugar-derivative nucleotide is according to formula (3):
wherein A is selected from the group consisting of -N 3 , -C(O)R 3 , -(CH 2 ) i C¡C-R 4 , -SH, -SC(O)R 8 , -SC(O)OR 8 , -SC(S)OR 8 , -F, -Cl, -Br -I, -OS(O) 2 R 5 , terminal C 2 - C 24 alkenyl groups, C 3 - C 5 cycloalkenyl groups, C 4 - C 8 alkadienyl groups, terminal C 3 - C 24 allenyl groups and amino groups. The invention further relates to a glycoprotein obtainable by the process according to the invention, to a bioconjugate that can be obtained by conjugating the glycoprotein with a linker-conjugate, and to ²-(1,4)-N-acetylgalactosaminyltransferases that can be used in preparing the the glycoprotein according to the invention.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>HUMAN NECESSITIES</subject><subject>HYGIENE</subject><subject>MEDICAL OR VETERINARY SCIENCE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2024</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjLEKwjAURbs4iPoPb1SwQ23V-TV9aQNtUpKn0KkUiZNooX6KH2wGHdxc7oXLOXcevVprBDkH0ljgiqAxhZJKICujwUhAKOtOmIAxKQ0np3T5HV1Xs0XtJFl0FHRkUA7CU8iCrDpTAdKaJgg5McbrZJttYh2jIO7qEmsUbBw2Sv88LaPZdbhNfvXpRQSSWFSxHx-9n8bh4u_-2VObpvvsuDvkSfoH8gaj8UD7</recordid><startdate>20241030</startdate><enddate>20241030</enddate><creator>WIJDEVEN, Maria Antonia</creator><creator>VAN DELFT, Floris Louis</creator><creator>VAN BERKEL, Sander Sebastiaan</creator><creator>VAN GEEL, Remon</creator><scope>EVB</scope></search><sort><creationdate>20241030</creationdate><title>PROCESS FOR THE MODIFICATION OF A GLYCOPROTEIN USING A GLYCOSYLTRANSFERASE THAT IS OR IS DERIVED FROM A BETA-(1,4)-N-ACETYLGALACTOSAMINYLTRANSFERASE</title><author>WIJDEVEN, Maria Antonia ; VAN DELFT, Floris Louis ; VAN BERKEL, Sander Sebastiaan ; VAN GEEL, Remon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_EP3354726B13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre ; ger</language><creationdate>2024</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>HUMAN NECESSITIES</topic><topic>HYGIENE</topic><topic>MEDICAL OR VETERINARY SCIENCE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>WIJDEVEN, Maria Antonia</creatorcontrib><creatorcontrib>VAN DELFT, Floris Louis</creatorcontrib><creatorcontrib>VAN BERKEL, Sander Sebastiaan</creatorcontrib><creatorcontrib>VAN GEEL, Remon</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>WIJDEVEN, Maria Antonia</au><au>VAN DELFT, Floris Louis</au><au>VAN BERKEL, Sander Sebastiaan</au><au>VAN GEEL, Remon</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>PROCESS FOR THE MODIFICATION OF A GLYCOPROTEIN USING A GLYCOSYLTRANSFERASE THAT IS OR IS DERIVED FROM A BETA-(1,4)-N-ACETYLGALACTOSAMINYLTRANSFERASE</title><date>2024-10-30</date><risdate>2024</risdate><abstract>The present invention relates to a process for the enzymatic modification of a glycoprotein. The process comprises the step of contacting a glycoprotein comprising a glycan comprising a terminal GlcNAc-moiety, in the presence of glycosyltransferase that is, or is derived from, a ²-(1,4)-N-acetylgalactosaminyltransferase, with a non-natural sugar-derivative nucleotide. The non-natural sugar-derivative nucleotide is according to formula (3):
wherein A is selected from the group consisting of -N 3 , -C(O)R 3 , -(CH 2 ) i C¡C-R 4 , -SH, -SC(O)R 8 , -SC(O)OR 8 , -SC(S)OR 8 , -F, -Cl, -Br -I, -OS(O) 2 R 5 , terminal C 2 - C 24 alkenyl groups, C 3 - C 5 cycloalkenyl groups, C 4 - C 8 alkadienyl groups, terminal C 3 - C 24 allenyl groups and amino groups. The invention further relates to a glycoprotein obtainable by the process according to the invention, to a bioconjugate that can be obtained by conjugating the glycoprotein with a linker-conjugate, and to ²-(1,4)-N-acetylgalactosaminyltransferases that can be used in preparing the the glycoprotein according to the invention.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | PROCESS FOR THE MODIFICATION OF A GLYCOPROTEIN USING A GLYCOSYLTRANSFERASE THAT IS OR IS DERIVED FROM A BETA-(1,4)-N-ACETYLGALACTOSAMINYLTRANSFERASE |
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