NMR METHOD FOR QUANTITATIVELY DETERMINING AN ANALYTE IN A LIQUID SAMPLE USING A HYPERPOLARIZED GAS

NMR METHOD FOR QUANTITATIVELY DETERMINING AN ANALYTE IN A LIQUID SAMPLE USING A HYPERPOLARIZED GAS

The invention refers to a method for quantitatively determining an analyte in a liquid sample, comprising: providing a liquid sample comprising an analyte of interest and a marker substance bound to the analyte; preparing a mixed liquid sample by mixing the liquid sample with a hyperpolarized gas, t...

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Hauptverfasser: Mitschang, Lorenz, Korchak, Sergey, Kilian, Wolfgang
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Sprache:eng ; fre ; ger
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INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
MEASURING ELECTRIC VARIABLES
MEASURING MAGNETIC VARIABLES
PHYSICS
TESTING
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creator Mitschang, Lorenz
Korchak, Sergey
Kilian, Wolfgang
description The invention refers to a method for quantitatively determining an analyte in a liquid sample, comprising: providing a liquid sample comprising an analyte of interest and a marker substance bound to the analyte; preparing a mixed liquid sample by mixing the liquid sample with a hyperpolarized gas, the marker substance and the hyperpolarized gas providing a host-guest system in the mixed sample, wherein the hyperpolarized gas is having a shift of nuclear magnetic resonance frequencies in case of a transition from a freely dissolved state to a host-bound state in which the hyperpolarized gas is bound to the marker substance in the mixed sample; experimentally determining the mixed sample by nuclear magnetic resonance (NMR) spectroscopy; from results of the NMR spectroscopy, determining a concentration of the hyperpolarized gas in the mixed liquid sample for at least one of the freely dissolved state and the host-bound state of the hyperpolarized gas; and quantitatively determining the analyte in the liquid sample. Also, use of the method for quantitatively determining a liquid biological ex-vivo sample is provided. Further, it is referred to use of the method in a multiplexing mode for quantitation of a plurality of analytes by different markers used simultaneously.
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subjects INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
MEASURING ELECTRIC VARIABLES
MEASURING MAGNETIC VARIABLES
PHYSICS
TESTING
title NMR METHOD FOR QUANTITATIVELY DETERMINING AN ANALYTE IN A LIQUID SAMPLE USING A HYPERPOLARIZED GAS
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