CELL CULTURE METHOD AND CELL CULTURE DEVICE

It is an object of the present invention to provide a cell culture method and a cell culture apparatus capable of culturing cells for a long period of time without causing stress or damage to the cells. Therefore, the cell culture apparatus includes a supply port 4 provided at one end of a culture dish 3, a discharge port 5 provided at the other end of the culture dish 3 so as to sandwich cultured cells 6 between the supply port 4 and the discharge port 5, a reservoir tank 11 that stores a culture liquid to be supplied to the culture dish 3, a waste liquid tank 21 that stores the culture liquid to be discharged from the culture dish, a supply-side micro flow rate pump 12 that supplies the culture liquid in the reservoir tank 11 to the culture dish 3 through the supply port 4, a discharge-side micro flow rate pump 22 that discharges the culture liquid from the culture dish 3 through the discharge port 5, and a flow rate controller 30 that performs flow rate control of the supply-side micro flow rate pump 12 and the discharge-side micro flow rate pump 22 so that a moving linear velocity of the culture liquid from the supply port 4 toward the discharge port 5 is less than a maximum velocity at which shear stress is not applied to the cultured cells.