METHOD FOR PERFORMING A SAMPLE ASSAY WITH A MICROFLUIDIC CARTRIDGES WITH INTEGRATED ASSAY CONTROLS

Disclosed is a microassay testing system, including a microfluidic cartridge and a compact microprocessor-controlled instrument for fluorometric assays in liquid samples, the cartridge having integrated process controls and positive and negative assay controls. The instrument has a scanning detector...

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Bibliographische Detailangaben
Hauptverfasser:	COUCH, Megan, HOFFMAN, Daniel, KOLB, Andrew, BERGDAHL, Asa, KAY, Justin, L, BRAGD, Matthew Scott, RIVERA, Rafael, LOFQUIST, Alan, K
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PHYSICS TESTING TRANSPORTING
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Beschreibung
Zusammenfassung:	Disclosed is a microassay testing system, including a microfluidic cartridge and a compact microprocessor-controlled instrument for fluorometric assays in liquid samples, the cartridge having integrated process controls and positive and negative assay controls. The instrument has a scanning detector head incorporating multiple optical channels. In a preferred configuration, the assay is validated using dual channel optics for monitoring a first fluorophore associated with a target analyte and a second fluorophore associated with a process control. Integrated positive and negative assay controls provide enhanced assay validation capabilities and facilitate analysis of test results. Applications include molecular biological assays based on PCR amplification of target nucleic acids and fluorometric assays in general.