MELTING CURVE ANALYSIS USING PNA PROBE, METHOD AND KIT FOR ANALYZING NUCLEOTIDE POLYMORPHISM USING MELTING CURVE ANALYSIS

Provided are a PNA probe for detecting nucleotide polymorphism of a target gene, a melting curve analysis method for detecting the nucleotide polymorphism of the target gene using the same, a nucleotide polymorphism analysis method of a target gene including the melting curve analysis method, and a...

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Hauptverfasser: KIM, Yongtae, KIM, Su Nam, PARK, Heekyung, KIM, Sung-Kee, KIM, Se Ryun, PARK, Chwang Siek, JOE, Goon Ho
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creator KIM, Yongtae
KIM, Su Nam
PARK, Heekyung
KIM, Sung-Kee
KIM, Se Ryun
PARK, Chwang Siek
JOE, Goon Ho
description Provided are a PNA probe for detecting nucleotide polymorphism of a target gene, a melting curve analysis method for detecting the nucleotide polymorphism of the target gene using the same, a nucleotide polymorphism analysis method of a target gene including the melting curve analysis method, and a kit for detecting the nucleotide polymorphism of the target gene containing the PNA probe. It is characterized that the PNA probe according to the present invention contains negative charge molecules. The modified PNA probe according to the present invention contains the negative charge molecules to have a high recognition ability with respect to a target DNA and a high coupling ability to the target DNA and to be rapidly dissociated by heat, such that the nucleotide polymorphism analysis may be relatively easily performed even in a heterozygous sample showing two melting curve graphs, and two or more adjacent single nucleotide polymorphisms may be simultaneously analyzed.
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
title MELTING CURVE ANALYSIS USING PNA PROBE, METHOD AND KIT FOR ANALYZING NUCLEOTIDE POLYMORPHISM USING MELTING CURVE ANALYSIS
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