MELTING CURVE ANALYSIS USING PNA PROBE, METHOD AND KIT FOR ANALYZING NUCLEOTIDE POLYMORPHISM USING MELTING CURVE ANALYSIS
Provided are a PNA probe for detecting nucleotide polymorphism of a target gene, a melting curve analysis method for detecting the nucleotide polymorphism of the target gene using the same, a nucleotide polymorphism analysis method of a target gene including the melting curve analysis method, and a...
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creator | KIM, Yongtae KIM, Su Nam PARK, Heekyung KIM, Sung-Kee KIM, Se Ryun PARK, Chwang Siek JOE, Goon Ho |
description | Provided are a PNA probe for detecting nucleotide polymorphism of a target gene, a melting curve analysis method for detecting the nucleotide polymorphism of the target gene using the same, a nucleotide polymorphism analysis method of a target gene including the melting curve analysis method, and a kit for detecting the nucleotide polymorphism of the target gene containing the PNA probe. It is characterized that the PNA probe according to the present invention contains negative charge molecules. The modified PNA probe according to the present invention contains the negative charge molecules to have a high recognition ability with respect to a target DNA and a high coupling ability to the target DNA and to be rapidly dissociated by heat, such that the nucleotide polymorphism analysis may be relatively easily performed even in a heterozygous sample showing two melting curve graphs, and two or more adjacent single nucleotide polymorphisms may be simultaneously analyzed. |
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It is characterized that the PNA probe according to the present invention contains negative charge molecules. The modified PNA probe according to the present invention contains the negative charge molecules to have a high recognition ability with respect to a target DNA and a high coupling ability to the target DNA and to be rapidly dissociated by heat, such that the nucleotide polymorphism analysis may be relatively easily performed even in a heterozygous sample showing two melting curve graphs, and two or more adjacent single nucleotide polymorphisms may be simultaneously analyzed.</description><language>eng ; fre ; ger</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2017</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20171101&DB=EPODOC&CC=EP&NR=3126511A4$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,777,882,25545,76296</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20171101&DB=EPODOC&CC=EP&NR=3126511A4$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>KIM, Yongtae</creatorcontrib><creatorcontrib>KIM, Su Nam</creatorcontrib><creatorcontrib>PARK, Heekyung</creatorcontrib><creatorcontrib>KIM, Sung-Kee</creatorcontrib><creatorcontrib>KIM, Se Ryun</creatorcontrib><creatorcontrib>PARK, Chwang Siek</creatorcontrib><creatorcontrib>JOE, Goon Ho</creatorcontrib><title>MELTING CURVE ANALYSIS USING PNA PROBE, METHOD AND KIT FOR ANALYZING NUCLEOTIDE POLYMORPHISM USING MELTING CURVE ANALYSIS</title><description>Provided are a PNA probe for detecting nucleotide polymorphism of a target gene, a melting curve analysis method for detecting the nucleotide polymorphism of the target gene using the same, a nucleotide polymorphism analysis method of a target gene including the melting curve analysis method, and a kit for detecting the nucleotide polymorphism of the target gene containing the PNA probe. It is characterized that the PNA probe according to the present invention contains negative charge molecules. The modified PNA probe according to the present invention contains the negative charge molecules to have a high recognition ability with respect to a target DNA and a high coupling ability to the target DNA and to be rapidly dissociated by heat, such that the nucleotide polymorphism analysis may be relatively easily performed even in a heterozygous sample showing two melting curve graphs, and two or more adjacent single nucleotide polymorphisms may be simultaneously analyzed.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2017</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZKj0dfUJ8fRzV3AODQpzVXD0c_SJDPYMVggNBgkG-DkqBAT5O7nqKPi6hnj4uwAVuCh4e4YouPkHQRRHgdT5hTr7uPqHeLq4KgT4-0T6-gcFeHgG-0JNwW4FDwNrWmJOcSovlOZmUHBzDXH20E0tyI9PLS5ITE7NSy2Jdw0wNjQyMzU0dDQxJkIJAHzcOiQ</recordid><startdate>20171101</startdate><enddate>20171101</enddate><creator>KIM, Yongtae</creator><creator>KIM, Su Nam</creator><creator>PARK, Heekyung</creator><creator>KIM, Sung-Kee</creator><creator>KIM, Se Ryun</creator><creator>PARK, Chwang Siek</creator><creator>JOE, Goon Ho</creator><scope>EVB</scope></search><sort><creationdate>20171101</creationdate><title>MELTING CURVE ANALYSIS USING PNA PROBE, METHOD AND KIT FOR ANALYZING NUCLEOTIDE POLYMORPHISM USING MELTING CURVE ANALYSIS</title><author>KIM, Yongtae ; KIM, Su Nam ; PARK, Heekyung ; KIM, Sung-Kee ; KIM, Se Ryun ; PARK, Chwang Siek ; JOE, Goon Ho</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_EP3126511A43</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre ; ger</language><creationdate>2017</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>KIM, Yongtae</creatorcontrib><creatorcontrib>KIM, Su Nam</creatorcontrib><creatorcontrib>PARK, Heekyung</creatorcontrib><creatorcontrib>KIM, Sung-Kee</creatorcontrib><creatorcontrib>KIM, Se Ryun</creatorcontrib><creatorcontrib>PARK, Chwang Siek</creatorcontrib><creatorcontrib>JOE, Goon Ho</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>KIM, Yongtae</au><au>KIM, Su Nam</au><au>PARK, Heekyung</au><au>KIM, Sung-Kee</au><au>KIM, Se Ryun</au><au>PARK, Chwang Siek</au><au>JOE, Goon Ho</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>MELTING CURVE ANALYSIS USING PNA PROBE, METHOD AND KIT FOR ANALYZING NUCLEOTIDE POLYMORPHISM USING MELTING CURVE ANALYSIS</title><date>2017-11-01</date><risdate>2017</risdate><abstract>Provided are a PNA probe for detecting nucleotide polymorphism of a target gene, a melting curve analysis method for detecting the nucleotide polymorphism of the target gene using the same, a nucleotide polymorphism analysis method of a target gene including the melting curve analysis method, and a kit for detecting the nucleotide polymorphism of the target gene containing the PNA probe. It is characterized that the PNA probe according to the present invention contains negative charge molecules. The modified PNA probe according to the present invention contains the negative charge molecules to have a high recognition ability with respect to a target DNA and a high coupling ability to the target DNA and to be rapidly dissociated by heat, such that the nucleotide polymorphism analysis may be relatively easily performed even in a heterozygous sample showing two melting curve graphs, and two or more adjacent single nucleotide polymorphisms may be simultaneously analyzed.</abstract><oa>free_for_read</oa></addata></record> |
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subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
title | MELTING CURVE ANALYSIS USING PNA PROBE, METHOD AND KIT FOR ANALYZING NUCLEOTIDE POLYMORPHISM USING MELTING CURVE ANALYSIS |
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