NUCLEIC ACID DETECTION AND ASSAY METHOD USING MASK OLIGONUCLEOTIDE, AND DEVICE FOR SAME

Very simple, highly sensitive detection or quantification of target nucleic acids of interest has been achieved by: hybridizing mask oligonucleotides to regions in a single-stranded region of a nucleic acid to be assayed between which a region to be hybridized by an oligonucleotide probe is position...

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Hauptverfasser: SUGIMOTO, Norihiko, ABE, Kanako, KAMEI, Kazumasa, EDA, Souji, UESAKA, Yoshihiko, SHIBAHARA, Yusuke, UEHARA, Hirotsugu, ASAKURA, Masahiro, OKU, Yuichi
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creator SUGIMOTO, Norihiko
ABE, Kanako
KAMEI, Kazumasa
EDA, Souji
UESAKA, Yoshihiko
SHIBAHARA, Yusuke
UEHARA, Hirotsugu
ASAKURA, Masahiro
OKU, Yuichi
description Very simple, highly sensitive detection or quantification of target nucleic acids of interest has been achieved by: hybridizing mask oligonucleotides to regions in a single-stranded region of a nucleic acid to be assayed between which a region to be hybridized by an oligonucleotide probe is positioned, thereby opening the probe-hybridizing region and keeping the single-stranded region of the target nucleic acid stable, and then subjecting this nucleic acid having the single-stranded region to nucleic acid chromatography.
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subjects APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
COMPOSITIONS THEREOF
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
CULTURE MEDIA
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title NUCLEIC ACID DETECTION AND ASSAY METHOD USING MASK OLIGONUCLEOTIDE, AND DEVICE FOR SAME
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