PROTEIN CRYSTALS
A method for producing an ordered protein lattice, the method comprising: (a) providing a first component comprising a subunit of a homooligomeric protein assembly fused to a first subunit of a heterooligomeric protein assembly, and a second component comprising a second subunit of the heterooligome...
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| creator | LEWIS, Ceri, John SINCLAIR, John, Charles |
| description | A method for producing an ordered protein lattice, the method comprising: (a) providing a first component comprising a subunit of a homooligomeric protein assembly fused to a first subunit of a heterooligomeric protein assembly, and a second component comprising a second subunit of the heterooligomeric protein assembly, wherein the homooligomeric protein assembly and the heterooligomeric protein assembly are each symmetrical in two or three dimensions and share a rotational symmetry axis of the same order; (b) mixing said first monomer and said second monomer to produce a mixture; and (c1) (i) heating the mixture to a temperature about 2° C. to about 10° C. below the visible dissociation temperature; (ii) cooling the mixture by about 10° C. to about 20° C.; and (iii) repeating steps (i) and (ii) at least 10 times; or (c2) (i) heating the mixture to a temperature about 2° C. to about 30° C. or more below the visible dissociation temperature; and (ii) holding the mixture at a temperature about 2° C. to about 30° C. or more below the melt temperature, thereby producing an ordered protein lattice. |
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(b) mixing said first monomer and said second monomer to produce a mixture; and (c1) (i) heating the mixture to a temperature about 2° C. to about 10° C. below the visible dissociation temperature; (ii) cooling the mixture by about 10° C. to about 20° C.; and (iii) repeating steps (i) and (ii) at least 10 times; or (c2) (i) heating the mixture to a temperature about 2° C. to about 30° C. or more below the visible dissociation temperature; and (ii) holding the mixture at a temperature about 2° C. to about 30° C. or more below the melt temperature, thereby producing an ordered protein lattice.</description><language>eng ; fre ; ger</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMES ; ENZYMOLOGY ; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES ; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; NANOTECHNOLOGY ; ORGANIC CHEMISTRY ; PEPTIDES ; PERFORMING OPERATIONS ; 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(b) mixing said first monomer and said second monomer to produce a mixture; and (c1) (i) heating the mixture to a temperature about 2° C. to about 10° C. below the visible dissociation temperature; (ii) cooling the mixture by about 10° C. to about 20° C.; and (iii) repeating steps (i) and (ii) at least 10 times; or (c2) (i) heating the mixture to a temperature about 2° C. to about 30° C. or more below the visible dissociation temperature; and (ii) holding the mixture at a temperature about 2° C. to about 30° C. or more below the melt temperature, thereby producing an ordered protein lattice.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMES</subject><subject>ENZYMOLOGY</subject><subject>MANUFACTURE OR TREATMENT OF NANOSTRUCTURES</subject><subject>MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>NANOTECHNOLOGY</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PERFORMING OPERATIONS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES</subject><subject>SPIRITS</subject><subject>TRANSPORTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2017</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZBAICPIPcfX0U3AOigwOcfQJ5mFgTUvMKU7lhdLcDApuriHOHrqpBfnxqcUFicmpeakl8a4BxgbGZsZGxk6GxkQoAQDb-B0_</recordid><startdate>20170816</startdate><enddate>20170816</enddate><creator>LEWIS, Ceri, John</creator><creator>SINCLAIR, John, Charles</creator><scope>EVB</scope></search><sort><creationdate>20170816</creationdate><title>PROTEIN CRYSTALS</title><author>LEWIS, Ceri, John ; 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(b) mixing said first monomer and said second monomer to produce a mixture; and (c1) (i) heating the mixture to a temperature about 2° C. to about 10° C. below the visible dissociation temperature; (ii) cooling the mixture by about 10° C. to about 20° C.; and (iii) repeating steps (i) and (ii) at least 10 times; or (c2) (i) heating the mixture to a temperature about 2° C. to about 30° C. or more below the visible dissociation temperature; and (ii) holding the mixture at a temperature about 2° C. to about 30° C. or more below the melt temperature, thereby producing an ordered protein lattice.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMES ENZYMOLOGY MANUFACTURE OR TREATMENT OF NANOSTRUCTURES MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NANOTECHNOLOGY ORGANIC CHEMISTRY PEPTIDES PERFORMING OPERATIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES SPIRITS TRANSPORTING VINEGAR WINE |
| title | PROTEIN CRYSTALS |
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