PLANTS RESISTANT TO PATHOGENIC MICROORGANISMS GROWING IN VASCULAR TISSUES
The present invention relates to the generation of transgenic plants resistant to infections caused by microorganisms restricted to the phloem, and it comprises the induction of the expression of a chimeric or fusion protein, which comprises a phloem protein of Citrus aurantifolia (CsPP16), a linker...
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| creator | XOCONOSTLE CÁZARES, Beatriz CHAVARIN PALACIO, Claudio GUERRA LUPIÁN, Miguel Ángel TRUJILLO ARRIAGA, Francisco Javier ZECUA NÁJERA, Rebeca GÓMEZ FELIPE, Andrea MORALES GALVÁN, Oscar LÓPEZ BUENFIL, José Abel RUÍZ MEDRANO, Roberto |
| description | The present invention relates to the generation of transgenic plants resistant to infections caused by microorganisms restricted to the phloem, and it comprises the induction of the expression of a chimeric or fusion protein, which comprises a phloem protein of Citrus aurantifolia (CsPP16), a linker protein and a protein with antimicrobial activity; for example, with antibacterial activity or antibacterial hydrolytic enzyme. The portion of the fusion protein corresponding to the CsPP16 protein can exert its function of simplasmic movement, which occurs via the plasmodesmata of the plant to reach the phloem sieve tubes, while the antimicrobial portion can act to eliminate the microorganism, which settles in the phloem. The invention allows controlling the disease that causes the citrus yellowing or Huanglongbing (HLB), also applying to the treatment of other diseases caused by bacteria and other microorganisms that settle in the vascular tissue of higher plants. The invention includes the designed molecular vectors carrying and expressing a fusion protein and the transgenic plants obtained with a stable expression of said fusion protein and the transformation method of adult plants, healthy or diseased, to express the chimeric proteins and controlling, e.g., HLB disease. The transformation of diseased plants with the fusion protein is a method to produce healthy shoots free from the microorganism causing the disease; e.g., free from HLB bacteria. |
| format | Patent |
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The portion of the fusion protein corresponding to the CsPP16 protein can exert its function of simplasmic movement, which occurs via the plasmodesmata of the plant to reach the phloem sieve tubes, while the antimicrobial portion can act to eliminate the microorganism, which settles in the phloem. The invention allows controlling the disease that causes the citrus yellowing or Huanglongbing (HLB), also applying to the treatment of other diseases caused by bacteria and other microorganisms that settle in the vascular tissue of higher plants. The invention includes the designed molecular vectors carrying and expressing a fusion protein and the transgenic plants obtained with a stable expression of said fusion protein and the transformation method of adult plants, healthy or diseased, to express the chimeric proteins and controlling, e.g., HLB disease. The transformation of diseased plants with the fusion protein is a method to produce healthy shoots free from the microorganism causing the disease; e.g., free from HLB bacteria.</description><language>eng ; fre ; ger</language><subject>AGRICULTURE ; ANIMAL HUSBANDRY ; BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FISHING ; FORESTRY ; HUMAN NECESSITIES ; HUNTING ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; NEW PLANTS OR PROCESSES FOR OBTAINING THEM ; ORGANIC CHEMISTRY ; PEPTIDES ; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TRAPPING ; VINEGAR ; WINE</subject><creationdate>2020</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20200812&DB=EPODOC&CC=EP&NR=2988590B1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25543,76293</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20200812&DB=EPODOC&CC=EP&NR=2988590B1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>XOCONOSTLE CÁZARES, Beatriz</creatorcontrib><creatorcontrib>CHAVARIN PALACIO, Claudio</creatorcontrib><creatorcontrib>GUERRA LUPIÁN, Miguel Ángel</creatorcontrib><creatorcontrib>TRUJILLO ARRIAGA, Francisco Javier</creatorcontrib><creatorcontrib>ZECUA NÁJERA, Rebeca</creatorcontrib><creatorcontrib>GÓMEZ FELIPE, Andrea</creatorcontrib><creatorcontrib>MORALES GALVÁN, Oscar</creatorcontrib><creatorcontrib>LÓPEZ BUENFIL, José Abel</creatorcontrib><creatorcontrib>RUÍZ MEDRANO, Roberto</creatorcontrib><title>PLANTS RESISTANT TO PATHOGENIC MICROORGANISMS GROWING IN VASCULAR TISSUES</title><description>The present invention relates to the generation of transgenic plants resistant to infections caused by microorganisms restricted to the phloem, and it comprises the induction of the expression of a chimeric or fusion protein, which comprises a phloem protein of Citrus aurantifolia (CsPP16), a linker protein and a protein with antimicrobial activity; for example, with antibacterial activity or antibacterial hydrolytic enzyme. The portion of the fusion protein corresponding to the CsPP16 protein can exert its function of simplasmic movement, which occurs via the plasmodesmata of the plant to reach the phloem sieve tubes, while the antimicrobial portion can act to eliminate the microorganism, which settles in the phloem. The invention allows controlling the disease that causes the citrus yellowing or Huanglongbing (HLB), also applying to the treatment of other diseases caused by bacteria and other microorganisms that settle in the vascular tissue of higher plants. The invention includes the designed molecular vectors carrying and expressing a fusion protein and the transgenic plants obtained with a stable expression of said fusion protein and the transformation method of adult plants, healthy or diseased, to express the chimeric proteins and controlling, e.g., HLB disease. The transformation of diseased plants with the fusion protein is a method to produce healthy shoots free from the microorganism causing the disease; e.g., free from HLB bacteria.</description><subject>AGRICULTURE</subject><subject>ANIMAL HUSBANDRY</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FISHING</subject><subject>FORESTRY</subject><subject>HUMAN NECESSITIES</subject><subject>HUNTING</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TRAPPING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2020</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZPAM8HH0CwlWCHIN9gwOATIVQvwVAhxDPPzdXf08nRV8PZ2D_P2D3B39PIN9gxXcg_zDPf3cFTz9FMIcg51DfRyDFEI8g4NDXYN5GFjTEnOKU3mhNDeDgptriLOHbmpBfnxqcUFicmpeakm8a4CRpYWFqaWBk6ExEUoAKD4tcg</recordid><startdate>20200812</startdate><enddate>20200812</enddate><creator>XOCONOSTLE CÁZARES, Beatriz</creator><creator>CHAVARIN PALACIO, Claudio</creator><creator>GUERRA LUPIÁN, Miguel Ángel</creator><creator>TRUJILLO ARRIAGA, Francisco Javier</creator><creator>ZECUA NÁJERA, Rebeca</creator><creator>GÓMEZ FELIPE, Andrea</creator><creator>MORALES GALVÁN, Oscar</creator><creator>LÓPEZ BUENFIL, José Abel</creator><creator>RUÍZ MEDRANO, Roberto</creator><scope>EVB</scope></search><sort><creationdate>20200812</creationdate><title>PLANTS RESISTANT TO PATHOGENIC MICROORGANISMS GROWING IN VASCULAR TISSUES</title><author>XOCONOSTLE CÁZARES, Beatriz ; CHAVARIN PALACIO, Claudio ; GUERRA LUPIÁN, Miguel Ángel ; TRUJILLO ARRIAGA, Francisco Javier ; ZECUA NÁJERA, Rebeca ; GÓMEZ FELIPE, Andrea ; MORALES GALVÁN, Oscar ; LÓPEZ BUENFIL, José Abel ; RUÍZ MEDRANO, Roberto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_EP2988590B13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre ; ger</language><creationdate>2020</creationdate><topic>AGRICULTURE</topic><topic>ANIMAL HUSBANDRY</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FISHING</topic><topic>FORESTRY</topic><topic>HUMAN NECESSITIES</topic><topic>HUNTING</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>TRAPPING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>XOCONOSTLE CÁZARES, Beatriz</creatorcontrib><creatorcontrib>CHAVARIN PALACIO, Claudio</creatorcontrib><creatorcontrib>GUERRA LUPIÁN, Miguel Ángel</creatorcontrib><creatorcontrib>TRUJILLO ARRIAGA, Francisco Javier</creatorcontrib><creatorcontrib>ZECUA NÁJERA, Rebeca</creatorcontrib><creatorcontrib>GÓMEZ FELIPE, Andrea</creatorcontrib><creatorcontrib>MORALES GALVÁN, Oscar</creatorcontrib><creatorcontrib>LÓPEZ BUENFIL, José Abel</creatorcontrib><creatorcontrib>RUÍZ MEDRANO, Roberto</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>XOCONOSTLE CÁZARES, Beatriz</au><au>CHAVARIN PALACIO, Claudio</au><au>GUERRA LUPIÁN, Miguel Ángel</au><au>TRUJILLO ARRIAGA, Francisco Javier</au><au>ZECUA NÁJERA, Rebeca</au><au>GÓMEZ FELIPE, Andrea</au><au>MORALES GALVÁN, Oscar</au><au>LÓPEZ BUENFIL, José Abel</au><au>RUÍZ MEDRANO, Roberto</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>PLANTS RESISTANT TO PATHOGENIC MICROORGANISMS GROWING IN VASCULAR TISSUES</title><date>2020-08-12</date><risdate>2020</risdate><abstract>The present invention relates to the generation of transgenic plants resistant to infections caused by microorganisms restricted to the phloem, and it comprises the induction of the expression of a chimeric or fusion protein, which comprises a phloem protein of Citrus aurantifolia (CsPP16), a linker protein and a protein with antimicrobial activity; for example, with antibacterial activity or antibacterial hydrolytic enzyme. The portion of the fusion protein corresponding to the CsPP16 protein can exert its function of simplasmic movement, which occurs via the plasmodesmata of the plant to reach the phloem sieve tubes, while the antimicrobial portion can act to eliminate the microorganism, which settles in the phloem. The invention allows controlling the disease that causes the citrus yellowing or Huanglongbing (HLB), also applying to the treatment of other diseases caused by bacteria and other microorganisms that settle in the vascular tissue of higher plants. The invention includes the designed molecular vectors carrying and expressing a fusion protein and the transgenic plants obtained with a stable expression of said fusion protein and the transformation method of adult plants, healthy or diseased, to express the chimeric proteins and controlling, e.g., HLB disease. The transformation of diseased plants with the fusion protein is a method to produce healthy shoots free from the microorganism causing the disease; e.g., free from HLB bacteria.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM ORGANIC CHEMISTRY PEPTIDES PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE |
| title | PLANTS RESISTANT TO PATHOGENIC MICROORGANISMS GROWING IN VASCULAR TISSUES |
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