Method and apparatus for disruption of biomass cells
The present invention relates to a method for disrupting suspended biomass material comprising the steps of: - providing a suspension of biomass material in a first vessel (1), said suspension having a dry cell weight being comprised between 0.1% and 15% (w/w); - pressurizing said suspension to a pr...
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| creator | ELST, Kathy D'HONDT, Els GARCIA-GONZALEZ, Linsey WIJFFELS, René H EPPINK, Michel H. M GÜNERKEN, Emre |
| description | The present invention relates to a method for disrupting suspended biomass material comprising the steps of:
- providing a suspension of biomass material in a first vessel (1), said suspension having a dry cell weight being comprised between 0.1% and 15% (w/w);
- pressurizing said suspension to a pressure (p1) being comprised between 7500 kPa and 450000 kPa by using a first high-pressure pump (10);
- providing a pressurized gas disruption agent and/or a pressurized liquid disruption agent in a second vessel (2);
- pressurizing said gas and/or liquid disruption agent to a pressure (p2) being comprised between 7500 kPa and 450000 kPa by using a second high-pressure pump (20), said pressure (p2) being higher than said pressure (p1);
- injecting a stream of said pressurized disruption agent into a stream of said pressurized suspension of biomass material by using a sparger system (7), thereby bringing said two streams together and forming a micro-emulsion;
- passing said micro-emulsion sequentially through a hydrodynamic agitation system (11) and a relief valve (13). An apparatus (and stack of (parallel) systems) for disrupting suspended biomass material and use thereof is provided as well. |
| format | Patent |
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- providing a suspension of biomass material in a first vessel (1), said suspension having a dry cell weight being comprised between 0.1% and 15% (w/w);
- pressurizing said suspension to a pressure (p1) being comprised between 7500 kPa and 450000 kPa by using a first high-pressure pump (10);
- providing a pressurized gas disruption agent and/or a pressurized liquid disruption agent in a second vessel (2);
- pressurizing said gas and/or liquid disruption agent to a pressure (p2) being comprised between 7500 kPa and 450000 kPa by using a second high-pressure pump (20), said pressure (p2) being higher than said pressure (p1);
- injecting a stream of said pressurized disruption agent into a stream of said pressurized suspension of biomass material by using a sparger system (7), thereby bringing said two streams together and forming a micro-emulsion;
- passing said micro-emulsion sequentially through a hydrodynamic agitation system (11) and a relief valve (13). An apparatus (and stack of (parallel) systems) for disrupting suspended biomass material and use thereof is provided as well.</description><language>eng ; fre ; ger</language><subject>APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY ; BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING ; MUTATION OR GENETIC ENGINEERING ; PERFORMING OPERATIONS ; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TRANSPORTING ; VINEGAR ; WINE</subject><creationdate>2017</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20170301&DB=EPODOC&CC=EP&NR=2977439B1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20170301&DB=EPODOC&CC=EP&NR=2977439B1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>ELST, Kathy</creatorcontrib><creatorcontrib>D'HONDT, Els</creatorcontrib><creatorcontrib>GARCIA-GONZALEZ, Linsey</creatorcontrib><creatorcontrib>WIJFFELS, René H</creatorcontrib><creatorcontrib>EPPINK, Michel H. M</creatorcontrib><creatorcontrib>GÜNERKEN, Emre</creatorcontrib><title>Method and apparatus for disruption of biomass cells</title><description>The present invention relates to a method for disrupting suspended biomass material comprising the steps of:
- providing a suspension of biomass material in a first vessel (1), said suspension having a dry cell weight being comprised between 0.1% and 15% (w/w);
- pressurizing said suspension to a pressure (p1) being comprised between 7500 kPa and 450000 kPa by using a first high-pressure pump (10);
- providing a pressurized gas disruption agent and/or a pressurized liquid disruption agent in a second vessel (2);
- pressurizing said gas and/or liquid disruption agent to a pressure (p2) being comprised between 7500 kPa and 450000 kPa by using a second high-pressure pump (20), said pressure (p2) being higher than said pressure (p1);
- injecting a stream of said pressurized disruption agent into a stream of said pressurized suspension of biomass material by using a sparger system (7), thereby bringing said two streams together and forming a micro-emulsion;
- passing said micro-emulsion sequentially through a hydrodynamic agitation system (11) and a relief valve (13). An apparatus (and stack of (parallel) systems) for disrupting suspended biomass material and use thereof is provided as well.</description><subject>APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PERFORMING OPERATIONS</subject><subject>PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TRANSPORTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2017</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZDDxTS3JyE9RSMwD4oKCxKLEktJihbT8IoWUzOKi0oKSzPw8hfw0haTM_NzE4mKF5NScnGIeBta0xJziVF4ozc2g4OYa4uyhm1qQH59aXJCYnJqXWhLvGmBkaW5uYmzpZGhMhBIA3hwsig</recordid><startdate>20170301</startdate><enddate>20170301</enddate><creator>ELST, Kathy</creator><creator>D'HONDT, Els</creator><creator>GARCIA-GONZALEZ, Linsey</creator><creator>WIJFFELS, René H</creator><creator>EPPINK, Michel H. M</creator><creator>GÜNERKEN, Emre</creator><scope>EVB</scope></search><sort><creationdate>20170301</creationdate><title>Method and apparatus for disruption of biomass cells</title><author>ELST, Kathy ; D'HONDT, Els ; GARCIA-GONZALEZ, Linsey ; WIJFFELS, René H ; EPPINK, Michel H. M ; GÜNERKEN, Emre</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_EP2977439B13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre ; ger</language><creationdate>2017</creationdate><topic>APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PERFORMING OPERATIONS</topic><topic>PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>TRANSPORTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>ELST, Kathy</creatorcontrib><creatorcontrib>D'HONDT, Els</creatorcontrib><creatorcontrib>GARCIA-GONZALEZ, Linsey</creatorcontrib><creatorcontrib>WIJFFELS, René H</creatorcontrib><creatorcontrib>EPPINK, Michel H. M</creatorcontrib><creatorcontrib>GÜNERKEN, Emre</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>ELST, Kathy</au><au>D'HONDT, Els</au><au>GARCIA-GONZALEZ, Linsey</au><au>WIJFFELS, René H</au><au>EPPINK, Michel H. M</au><au>GÜNERKEN, Emre</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method and apparatus for disruption of biomass cells</title><date>2017-03-01</date><risdate>2017</risdate><abstract>The present invention relates to a method for disrupting suspended biomass material comprising the steps of:
- providing a suspension of biomass material in a first vessel (1), said suspension having a dry cell weight being comprised between 0.1% and 15% (w/w);
- pressurizing said suspension to a pressure (p1) being comprised between 7500 kPa and 450000 kPa by using a first high-pressure pump (10);
- providing a pressurized gas disruption agent and/or a pressurized liquid disruption agent in a second vessel (2);
- pressurizing said gas and/or liquid disruption agent to a pressure (p2) being comprised between 7500 kPa and 450000 kPa by using a second high-pressure pump (20), said pressure (p2) being higher than said pressure (p1);
- injecting a stream of said pressurized disruption agent into a stream of said pressurized suspension of biomass material by using a sparger system (7), thereby bringing said two streams together and forming a micro-emulsion;
- passing said micro-emulsion sequentially through a hydrodynamic agitation system (11) and a relief valve (13). An apparatus (and stack of (parallel) systems) for disrupting suspended biomass material and use thereof is provided as well.</abstract><oa>free_for_read</oa></addata></record> |
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| language | eng ; fre ; ger |
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| subjects | APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRANSPORTING VINEGAR WINE |
| title | Method and apparatus for disruption of biomass cells |
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