METHOD OF NORMALIZING A FLUORESCENCE ANALYZER

METHOD OF NORMALIZING A FLUORESCENCE ANALYZER

A method of normalizing an analyzer response value of a fluorescence analyzer is provided. The method includes measuring an excitation spectrum of the analyzer and measuring an emission sensitivity spectrum of the analyzer. Next, a normalization factor based at least in part upon the excitation spec...

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Hauptverfasser: MISENER, GARLAND, CHRISTIAN, MILAN, JAMES, EDWARD, LACHAPELLE, ROBERT, W
Format: Patent
Sprache:eng ; fre ; ger
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COLORIMETRY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT,POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRA-RED,VISIBLE OR ULTRA-VIOLET LIGHT
MEASURING
PHYSICS
RADIATION PYROMETRY
TESTING
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creator MISENER, GARLAND, CHRISTIAN
MILAN, JAMES, EDWARD
LACHAPELLE, ROBERT, W
description A method of normalizing an analyzer response value of a fluorescence analyzer is provided. The method includes measuring an excitation spectrum of the analyzer and measuring an emission sensitivity spectrum of the analyzer. Next, a normalization factor based at least in part upon the excitation spectrum of the analyzer and the emission sensitivity spectrum of the analyzer is determined. The sample is then analyzed to obtain an uncorrected analyzer response value. A normalized analyzer response value is calculated based at least in part upon the uncorrected analyzer response value and the normalization factor.
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subjects COLORIMETRY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT,POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRA-RED,VISIBLE OR ULTRA-VIOLET LIGHT
MEASURING
PHYSICS
RADIATION PYROMETRY
TESTING
title METHOD OF NORMALIZING A FLUORESCENCE ANALYZER
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