Preparation of templates for nucleic acid sequencing
The present invention relates to a method of generating a template for a nucleic acid sequencing reaction comprising, (i) providing at least one double-stranded nucleic acid molecule attached to a solid support at the 5' end, (ii) cleaving one or both strands of the double-stranded nucleic acid...
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| creator | RIGATTI, ROBERTO RASOLONJATOVO, ISABELLE MARIE J MILTON, JOHN SMITH, GEOFFREY PAUL EARNSHAW, DAVID JAMES OST, TOBIAS WILLIAM BARR LIU, XIAOHAI TURCATTI, GERARDO WORSLEY, GRAHAM JOHN WU, XIAOLIN BARNES, COLIN ROMIEU, ANTHONY |
| description | The present invention relates to a method of generating a template for a nucleic acid sequencing reaction comprising,
(i) providing at least one double-stranded nucleic acid molecule attached to a solid support at the 5' end,
(ii) cleaving one or both strands of the double-stranded nucleic acid molecule at an abasic site, and
(iii) subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction. |
| format | Patent |
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(i) providing at least one double-stranded nucleic acid molecule attached to a solid support at the 5' end,
(ii) cleaving one or both strands of the double-stranded nucleic acid molecule at an abasic site, and
(iii) subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.</description><language>eng ; fre ; ger</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2011</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20110323&DB=EPODOC&CC=EP&NR=2298930A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25543,76293</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20110323&DB=EPODOC&CC=EP&NR=2298930A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>RIGATTI, ROBERTO</creatorcontrib><creatorcontrib>RASOLONJATOVO, ISABELLE MARIE J</creatorcontrib><creatorcontrib>MILTON, JOHN</creatorcontrib><creatorcontrib>SMITH, GEOFFREY PAUL</creatorcontrib><creatorcontrib>EARNSHAW, DAVID JAMES</creatorcontrib><creatorcontrib>OST, TOBIAS WILLIAM BARR</creatorcontrib><creatorcontrib>LIU, XIAOHAI</creatorcontrib><creatorcontrib>TURCATTI, GERARDO</creatorcontrib><creatorcontrib>WORSLEY, GRAHAM JOHN</creatorcontrib><creatorcontrib>WU, XIAOLIN</creatorcontrib><creatorcontrib>BARNES, COLIN</creatorcontrib><creatorcontrib>ROMIEU, ANTHONY</creatorcontrib><title>Preparation of templates for nucleic acid sequencing</title><description>The present invention relates to a method of generating a template for a nucleic acid sequencing reaction comprising,
(i) providing at least one double-stranded nucleic acid molecule attached to a solid support at the 5' end,
(ii) cleaving one or both strands of the double-stranded nucleic acid molecule at an abasic site, and
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(i) providing at least one double-stranded nucleic acid molecule attached to a solid support at the 5' end,
(ii) cleaving one or both strands of the double-stranded nucleic acid molecule at an abasic site, and
(iii) subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.</abstract><oa>free_for_read</oa></addata></record> |
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| language | eng ; fre ; ger |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | Preparation of templates for nucleic acid sequencing |
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