METHOD FOR FILTERING NUCLEIC ACIDS, IN PARTICULAR FROM FIXED TISSUE

The invention relates to a method for filtering nucleic acids, to a kit for carrying out the method according to the invention and to a novel use of magnetic particles for filtering a biological sample. The method according to the invention comprises the following steps: a) the sample is held in an...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: BOHMANN, KERSTIN, HENNIG, GUIDO, EUTING, HEIKE
Format: Patent
Sprache:eng ; fre ; ger
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Beschreibung
Zusammenfassung:The invention relates to a method for filtering nucleic acids, to a kit for carrying out the method according to the invention and to a novel use of magnetic particles for filtering a biological sample. The method according to the invention comprises the following steps: a) the sample is held in an aqueous solution; b) lysing of the sample; c) separation of cellular debris; and d) the nucleic acids are isolated from the solution, steps (a) to (c) taking place under non-chaotropic conditions.