Fractionation of protein containing mixtures

A primary aspect of the present invention relates to a method for the fractionation of a protein-containing mixture, said method comprising the steps of: a) optionally adjusting the pH of the mixture; b) applying said mixture to an expanded bed adsorption column comprising an adsorbent, said adsorbe...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	ANDERSEN, INGA VAARST, LIHME, ALLAN OTTO FOG, OLANDER, MORTEN AAE, HANSEN, MARIE BENDIX
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY CHEMISTRY FOODS OR FOODSTUFFS HUMAN NECESSITIES METALLURGY ORGANIC CHEMISTRY PEPTIDES PERFORMING OPERATIONS PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PROTEIN COMPOSITIONS FOR FOODSTUFFS SEPARATION THEIR RELEVANT APPARATUS THEIR TREATMENT, NOT COVERED BY OTHER CLASSES TRANSPORTING WORKING-UP PROTEINS FOR FOODSTUFFS
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Beschreibung
Zusammenfassung:	A primary aspect of the present invention relates to a method for the fractionation of a protein-containing mixture, said method comprising the steps of: a) optionally adjusting the pH of the mixture; b) applying said mixture to an expanded bed adsorption column comprising an adsorbent, said adsorbent comprises a particle with at least one high-density non-porous core, surrounded by a porous material; the absorbent having a particle density of at least 1.5g/ml, wherein said high-density non-porous core has a density of at least 4 g/ml, and the degree of expansion (H/H0) of the adsorbent is in the range of 1.2-5, and wherein the flow rate of applying said protein-containing mixture is at least 8 cm/min; c) optionally washing the column; d) eluting at least one protein from the adsorbent.