A METHOD FOR IDENTIFYING A PORK CONTENT IN A FOOD

Pork-specific PCR assay is performed for Halal authentication, by detecting porcine DNA in food products. DNA from raw meat samples is extracted. The extracted DNA is tested using primers that react by amplifying pork DNA but not beef and chicken DNA. The real-time PCR assay is sensitive with a low...

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Hauptverfasser: SAZILI , AWIS QURNI, ABDUL RAHIM, RAHA, KHALID, FARIHAH LIYANA, AZMI , AIDA AZRINA, MUSTAFA, SHUHAIMI, B. CHE MAN, YAAKOB
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creator SAZILI , AWIS QURNI
ABDUL RAHIM, RAHA
KHALID, FARIHAH LIYANA
AZMI , AIDA AZRINA
MUSTAFA, SHUHAIMI
B. CHE MAN, YAAKOB
description Pork-specific PCR assay is performed for Halal authentication, by detecting porcine DNA in food products. DNA from raw meat samples is extracted. The extracted DNA is tested using primers that react by amplifying pork DNA but not beef and chicken DNA. The real-time PCR assay is sensitive with a low detection limit when using samples that can be obtained from food products. The methods described herein can have a sensitivity threshold as low as 0.001 ng pork DNA or lower, whereas convention techniques typically do not have a detection limit lower than 0.1 ng pork DNA.
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
COMPOSITIONS THEREOF
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
CULTURE MEDIA
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title A METHOD FOR IDENTIFYING A PORK CONTENT IN A FOOD
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