METHOD OF DETECTING INTERACTIONS BETWEEN PROTEIN COMPONENTS

METHOD OF DETECTING INTERACTIONS BETWEEN PROTEIN COMPONENTS

A method of detecting protein interactions in a biological sample. Protein components in a first aliquot of a sample are labelled with a first bifunctional dye which cross-links any-interacting components. A second aliquot of said sample functions as a control sample, in which protein components are...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: HAGNER-MCWHIRTER, AASA, EDLUND, SOFIA, NEU, HENRIK
Format: Patent
Sprache:eng ; fre ; ger
Schlagworte:
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue
container_start_page
container_title
container_volume
creator HAGNER-MCWHIRTER, AASA
EDLUND, SOFIA
NEU, HENRIK
description A method of detecting protein interactions in a biological sample. Protein components in a first aliquot of a sample are labelled with a first bifunctional dye which cross-links any-interacting components. A second aliquot of said sample functions as a control sample, in which protein components are labelled with a different, mono functional dye. Thereafter, the two aliquots are mixed and all components are separated by electrophoresis. Finally, differences in luminescence of the separated dye-labelled components are detected. The two dyes should match one another with regard to charge and/or molecular weight but should emit different kinds of fluorescent light.
format Patent
fullrecord <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_EP2044440A1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>EP2044440A1</sourcerecordid><originalsourceid>FETCH-epo_espacenet_EP2044440A13</originalsourceid><addsrcrecordid>eNrjZLD2dQ3x8HdR8HdTcHENcXUO8fRzV_D0C3ENcgSy_f2CFZxcQ8JdXf0UAoL8Q1w9_RSc_X0D_P1c_UKCeRhY0xJzilN5oTQ3g4Kba4izh25qQX58anFBYnJqXmpJvGuAkYEJEBg4GhoToQQAptkpHw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>METHOD OF DETECTING INTERACTIONS BETWEEN PROTEIN COMPONENTS</title><source>esp@cenet</source><creator>HAGNER-MCWHIRTER, AASA ; EDLUND, SOFIA ; NEU, HENRIK</creator><creatorcontrib>HAGNER-MCWHIRTER, AASA ; EDLUND, SOFIA ; NEU, HENRIK</creatorcontrib><description>A method of detecting protein interactions in a biological sample. Protein components in a first aliquot of a sample are labelled with a first bifunctional dye which cross-links any-interacting components. A second aliquot of said sample functions as a control sample, in which protein components are labelled with a different, mono functional dye. Thereafter, the two aliquots are mixed and all components are separated by electrophoresis. Finally, differences in luminescence of the separated dye-labelled components are detected. The two dyes should match one another with regard to charge and/or molecular weight but should emit different kinds of fluorescent light.</description><language>eng ; fre ; ger</language><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; PHYSICS ; TESTING</subject><creationdate>2009</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20090408&amp;DB=EPODOC&amp;CC=EP&amp;NR=2044440A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20090408&amp;DB=EPODOC&amp;CC=EP&amp;NR=2044440A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>HAGNER-MCWHIRTER, AASA</creatorcontrib><creatorcontrib>EDLUND, SOFIA</creatorcontrib><creatorcontrib>NEU, HENRIK</creatorcontrib><title>METHOD OF DETECTING INTERACTIONS BETWEEN PROTEIN COMPONENTS</title><description>A method of detecting protein interactions in a biological sample. Protein components in a first aliquot of a sample are labelled with a first bifunctional dye which cross-links any-interacting components. A second aliquot of said sample functions as a control sample, in which protein components are labelled with a different, mono functional dye. Thereafter, the two aliquots are mixed and all components are separated by electrophoresis. Finally, differences in luminescence of the separated dye-labelled components are detected. The two dyes should match one another with regard to charge and/or molecular weight but should emit different kinds of fluorescent light.</description><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>PHYSICS</subject><subject>TESTING</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2009</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZLD2dQ3x8HdR8HdTcHENcXUO8fRzV_D0C3ENcgSy_f2CFZxcQ8JdXf0UAoL8Q1w9_RSc_X0D_P1c_UKCeRhY0xJzilN5oTQ3g4Kba4izh25qQX58anFBYnJqXmpJvGuAkYEJEBg4GhoToQQAptkpHw</recordid><startdate>20090408</startdate><enddate>20090408</enddate><creator>HAGNER-MCWHIRTER, AASA</creator><creator>EDLUND, SOFIA</creator><creator>NEU, HENRIK</creator><scope>EVB</scope></search><sort><creationdate>20090408</creationdate><title>METHOD OF DETECTING INTERACTIONS BETWEEN PROTEIN COMPONENTS</title><author>HAGNER-MCWHIRTER, AASA ; EDLUND, SOFIA ; NEU, HENRIK</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_EP2044440A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre ; ger</language><creationdate>2009</creationdate><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>PHYSICS</topic><topic>TESTING</topic><toplevel>online_resources</toplevel><creatorcontrib>HAGNER-MCWHIRTER, AASA</creatorcontrib><creatorcontrib>EDLUND, SOFIA</creatorcontrib><creatorcontrib>NEU, HENRIK</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>HAGNER-MCWHIRTER, AASA</au><au>EDLUND, SOFIA</au><au>NEU, HENRIK</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>METHOD OF DETECTING INTERACTIONS BETWEEN PROTEIN COMPONENTS</title><date>2009-04-08</date><risdate>2009</risdate><abstract>A method of detecting protein interactions in a biological sample. Protein components in a first aliquot of a sample are labelled with a first bifunctional dye which cross-links any-interacting components. A second aliquot of said sample functions as a control sample, in which protein components are labelled with a different, mono functional dye. Thereafter, the two aliquots are mixed and all components are separated by electrophoresis. Finally, differences in luminescence of the separated dye-labelled components are detected. The two dyes should match one another with regard to charge and/or molecular weight but should emit different kinds of fluorescent light.</abstract><oa>free_for_read</oa></addata></record>
fulltext fulltext_linktorsrc
identifier
ispartof
issn
language eng ; fre ; ger
recordid cdi_epo_espacenet_EP2044440A1
source esp@cenet
subjects INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
title METHOD OF DETECTING INTERACTIONS BETWEEN PROTEIN COMPONENTS
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-31T00%3A39%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=HAGNER-MCWHIRTER,%20AASA&rft.date=2009-04-08&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3EEP2044440A1%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true