METHOD FOR PRE-TREATMENT OF SERUM FOR ANALYSIS OF SUGAR CHAIN

A means for pre-treatment in glycomic analysis of a glycoprotein is provided by the present invention. A salt of the general formula (I): wherein Z, X, R 1 , R 2 , M, m and n are the same as described in DESCRIPTION, is useful as a protein solubilizer, and an oligosaccharide is efficiently released...

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Bibliographische Detailangaben
Hauptverfasser:	KITA, YOKO, FURUKAWA, JUN-ICHI, NISHIMURA, SHIN-ICHIRO, NAKANO, MIKA, TAKIMOTO, AKIO, MIURA, YOSHIAKI, SHINOHARA, YASURO
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	ACYCLIC OR CARBOCYCLIC COMPOUNDS BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	A means for pre-treatment in glycomic analysis of a glycoprotein is provided by the present invention. A salt of the general formula (I): wherein Z, X, R 1 , R 2 , M, m and n are the same as described in DESCRIPTION, is useful as a protein solubilizer, and an oligosaccharide is efficiently released from a sample if reductive alkylation and /or digestion by a proteinase are carried out under the presence of the said solubilizer at the first step in the glycomic analysis of glycoprotein derived from a living body.