SELECTION SYSTEM CONTAINING NON-ANTIBIOTIC RESISTANCE SELECTION MARKER

The present invention relates to a novel selection system, which is based on the use of an ara D gene, a mutated form of an ara D gene, a complementary sequence thereof, or a catalytically active fragment thereof as a selection marker and to the use of a bacterial strain deficient of the ara D gene....

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: TOOTS, URVE, MÄNNIK, ANDRES, USTAV, MART, TENSON, TANEL, ADOJAAN, MAARJA, LAHT, SILJA
Format: Patent
Sprache:eng ; fre ; ger
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Zusammenfassung:The present invention relates to a novel selection system, which is based on the use of an ara D gene, a mutated form of an ara D gene, a complementary sequence thereof, or a catalytically active fragment thereof as a selection marker and to the use of a bacterial strain deficient of the ara D gene. The present invention further relates to novel vectors containing an ara D gene, a mutated form of an ara D gene, a complementary sequence thereof, or a catalytically active fragment thereof and to novel bacterial strains deficient of the ara D gene. The present invention additionally relates to a method of selecting the cells transformed with a plasmid, which contains the gene of interest.