METHOD OF OBTAINING A RECOMBINANT MAMMALIAN MYELOMA CELL LINES ADAPTED TO GROWTH IN SERUM- AND PROTEIN-FREE MEDIA
The invention relates to a method of obtaining mammal cells which are adapted to grow in a medium free of serum and proteins. The inventive method includes a process consisting of two adaptation steps comprising a critical protein concentration interval for each cell line. The invention also relates...
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| creator | ROJAS DEL CALVO, LUIS VITORES SARAZOLA, SVIETA CASTILLO VITLLOCH, ADOLFO BOGGIANO AYO, TAMMY PEREZ RODRIGUEZ, ROLANDO |
| description | The invention relates to a method of obtaining mammal cells which are adapted to grow in a medium free of serum and proteins. The inventive method includes a process consisting of two adaptation steps comprising a critical protein concentration interval for each cell line. The invention also relates to mammal cell lines which grow in a stable manner in a medium free of serum and proteins over at least 40 generations. The invention further relates to clones which express anti-CD6 T1hT and EGFhR 3 antireceptor humanised antibodies and the anti-CD 3 T 3Q chimeric antibody as well as fragments of said antibodies. |
| format | Patent |
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| language | eng ; fre ; ger |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | METHOD OF OBTAINING A RECOMBINANT MAMMALIAN MYELOMA CELL LINES ADAPTED TO GROWTH IN SERUM- AND PROTEIN-FREE MEDIA |
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