MODULAR TRANSFECTION SYSTEMS BASED ON NUCLEOPROTEIN FILAMENTS
The present invention relates to a method for transfection of cells using at least one protein capable of forming nucleoprotein filaments, wherein the protein is initially modified with at least one functional component which influences one or more steps of the transfection, the nucleic acid to be t...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Patent |
| Sprache: | eng ; fre ; ger |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | ORTMANN, BODO ALTROGGE, LUDGER HEIN, KATHARINA CHRISTINE, RAINER TURBANSKI, TAMARA LORBACH, ELKE MALES, TATJANA GREMSE, MARION KLAES, ANDREA RIEMEN, GUDULA HELFRICH, JULIANA SCHMIDT, HANNS-MARTIN SIEBENKOTTEN, GREGOR LENZ, DIETMAR BROSTERHUS, HELMUT |
| description | The present invention relates to a method for transfection of cells using at least one protein capable of forming nucleoprotein filaments, wherein the protein is initially modified with at least one functional component which influences one or more steps of the transfection, the nucleic acid to be transfected is then loaded with the modified protein, whereby the nucleic acid and the protein form a filament-like complex, and this complex is finally added to the cells to be transfected. The invention further relates to a transfection agent consisting of nucleoprotein filaments (NPF), with at least one nucleoprotein filament-forming protein being modified with at least one functional component for the transfection. Furthermore, the present invention relates to the use of the transfection agent according to the invention for producing a drug for gene therapeutic treatment of humans and animals. The present inventions also includes corresponding pharmaceutical preparations, especially for use in gene therapy as well as the use of such transfection agents as component in kits. |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_EP1427837B1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>EP1427837B1</sourcerecordid><originalsourceid>FETCH-epo_espacenet_EP1427837B13</originalsourceid><addsrcrecordid>eNrjZLD19XcJ9XEMUggJcvQLdnN1DvH091MIjgwOcfUNVnByDHZ1UQAK-IU6-7j6BwT5h7h6-im4efo4-rr6hQTzMLCmJeYUp_JCaW4GBTfXEGcP3dSC_PjU4oLE5NS81JJ41wBDEyNzC2NzJ0NjIpQAABUaKfA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>MODULAR TRANSFECTION SYSTEMS BASED ON NUCLEOPROTEIN FILAMENTS</title><source>esp@cenet</source><creator>ORTMANN, BODO ; ALTROGGE, LUDGER ; HEIN, KATHARINA ; CHRISTINE, RAINER ; TURBANSKI, TAMARA ; LORBACH, ELKE ; MALES, TATJANA ; GREMSE, MARION ; KLAES, ANDREA ; RIEMEN, GUDULA ; HELFRICH, JULIANA ; SCHMIDT, HANNS-MARTIN ; SIEBENKOTTEN, GREGOR ; LENZ, DIETMAR ; BROSTERHUS, HELMUT</creator><creatorcontrib>ORTMANN, BODO ; ALTROGGE, LUDGER ; HEIN, KATHARINA ; CHRISTINE, RAINER ; TURBANSKI, TAMARA ; LORBACH, ELKE ; MALES, TATJANA ; GREMSE, MARION ; KLAES, ANDREA ; RIEMEN, GUDULA ; HELFRICH, JULIANA ; SCHMIDT, HANNS-MARTIN ; SIEBENKOTTEN, GREGOR ; LENZ, DIETMAR ; BROSTERHUS, HELMUT</creatorcontrib><description>The present invention relates to a method for transfection of cells using at least one protein capable of forming nucleoprotein filaments, wherein the protein is initially modified with at least one functional component which influences one or more steps of the transfection, the nucleic acid to be transfected is then loaded with the modified protein, whereby the nucleic acid and the protein form a filament-like complex, and this complex is finally added to the cells to be transfected. The invention further relates to a transfection agent consisting of nucleoprotein filaments (NPF), with at least one nucleoprotein filament-forming protein being modified with at least one functional component for the transfection. Furthermore, the present invention relates to the use of the transfection agent according to the invention for producing a drug for gene therapeutic treatment of humans and animals. The present inventions also includes corresponding pharmaceutical preparations, especially for use in gene therapy as well as the use of such transfection agents as component in kits.</description><language>eng ; fre ; ger</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; HUMAN NECESSITIES ; HYGIENE ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; MEDICAL OR VETERINARY SCIENCE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PHYSICS ; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TESTING ; VINEGAR ; WINE</subject><creationdate>2011</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20111026&DB=EPODOC&CC=EP&NR=1427837B1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20111026&DB=EPODOC&CC=EP&NR=1427837B1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>ORTMANN, BODO</creatorcontrib><creatorcontrib>ALTROGGE, LUDGER</creatorcontrib><creatorcontrib>HEIN, KATHARINA</creatorcontrib><creatorcontrib>CHRISTINE, RAINER</creatorcontrib><creatorcontrib>TURBANSKI, TAMARA</creatorcontrib><creatorcontrib>LORBACH, ELKE</creatorcontrib><creatorcontrib>MALES, TATJANA</creatorcontrib><creatorcontrib>GREMSE, MARION</creatorcontrib><creatorcontrib>KLAES, ANDREA</creatorcontrib><creatorcontrib>RIEMEN, GUDULA</creatorcontrib><creatorcontrib>HELFRICH, JULIANA</creatorcontrib><creatorcontrib>SCHMIDT, HANNS-MARTIN</creatorcontrib><creatorcontrib>SIEBENKOTTEN, GREGOR</creatorcontrib><creatorcontrib>LENZ, DIETMAR</creatorcontrib><creatorcontrib>BROSTERHUS, HELMUT</creatorcontrib><title>MODULAR TRANSFECTION SYSTEMS BASED ON NUCLEOPROTEIN FILAMENTS</title><description>The present invention relates to a method for transfection of cells using at least one protein capable of forming nucleoprotein filaments, wherein the protein is initially modified with at least one functional component which influences one or more steps of the transfection, the nucleic acid to be transfected is then loaded with the modified protein, whereby the nucleic acid and the protein form a filament-like complex, and this complex is finally added to the cells to be transfected. The invention further relates to a transfection agent consisting of nucleoprotein filaments (NPF), with at least one nucleoprotein filament-forming protein being modified with at least one functional component for the transfection. Furthermore, the present invention relates to the use of the transfection agent according to the invention for producing a drug for gene therapeutic treatment of humans and animals. The present inventions also includes corresponding pharmaceutical preparations, especially for use in gene therapy as well as the use of such transfection agents as component in kits.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>HUMAN NECESSITIES</subject><subject>HYGIENE</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>MEDICAL OR VETERINARY SCIENCE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PHYSICS</subject><subject>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TESTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2011</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZLD19XcJ9XEMUggJcvQLdnN1DvH091MIjgwOcfUNVnByDHZ1UQAK-IU6-7j6BwT5h7h6-im4efo4-rr6hQTzMLCmJeYUp_JCaW4GBTfXEGcP3dSC_PjU4oLE5NS81JJ41wBDEyNzC2NzJ0NjIpQAABUaKfA</recordid><startdate>20111026</startdate><enddate>20111026</enddate><creator>ORTMANN, BODO</creator><creator>ALTROGGE, LUDGER</creator><creator>HEIN, KATHARINA</creator><creator>CHRISTINE, RAINER</creator><creator>TURBANSKI, TAMARA</creator><creator>LORBACH, ELKE</creator><creator>MALES, TATJANA</creator><creator>GREMSE, MARION</creator><creator>KLAES, ANDREA</creator><creator>RIEMEN, GUDULA</creator><creator>HELFRICH, JULIANA</creator><creator>SCHMIDT, HANNS-MARTIN</creator><creator>SIEBENKOTTEN, GREGOR</creator><creator>LENZ, DIETMAR</creator><creator>BROSTERHUS, HELMUT</creator><scope>EVB</scope></search><sort><creationdate>20111026</creationdate><title>MODULAR TRANSFECTION SYSTEMS BASED ON NUCLEOPROTEIN FILAMENTS</title><author>ORTMANN, BODO ; ALTROGGE, LUDGER ; HEIN, KATHARINA ; CHRISTINE, RAINER ; TURBANSKI, TAMARA ; LORBACH, ELKE ; MALES, TATJANA ; GREMSE, MARION ; KLAES, ANDREA ; RIEMEN, GUDULA ; HELFRICH, JULIANA ; SCHMIDT, HANNS-MARTIN ; SIEBENKOTTEN, GREGOR ; LENZ, DIETMAR ; BROSTERHUS, HELMUT</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_EP1427837B13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre ; ger</language><creationdate>2011</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>HUMAN NECESSITIES</topic><topic>HYGIENE</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>MEDICAL OR VETERINARY SCIENCE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PHYSICS</topic><topic>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>TESTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>ORTMANN, BODO</creatorcontrib><creatorcontrib>ALTROGGE, LUDGER</creatorcontrib><creatorcontrib>HEIN, KATHARINA</creatorcontrib><creatorcontrib>CHRISTINE, RAINER</creatorcontrib><creatorcontrib>TURBANSKI, TAMARA</creatorcontrib><creatorcontrib>LORBACH, ELKE</creatorcontrib><creatorcontrib>MALES, TATJANA</creatorcontrib><creatorcontrib>GREMSE, MARION</creatorcontrib><creatorcontrib>KLAES, ANDREA</creatorcontrib><creatorcontrib>RIEMEN, GUDULA</creatorcontrib><creatorcontrib>HELFRICH, JULIANA</creatorcontrib><creatorcontrib>SCHMIDT, HANNS-MARTIN</creatorcontrib><creatorcontrib>SIEBENKOTTEN, GREGOR</creatorcontrib><creatorcontrib>LENZ, DIETMAR</creatorcontrib><creatorcontrib>BROSTERHUS, HELMUT</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>ORTMANN, BODO</au><au>ALTROGGE, LUDGER</au><au>HEIN, KATHARINA</au><au>CHRISTINE, RAINER</au><au>TURBANSKI, TAMARA</au><au>LORBACH, ELKE</au><au>MALES, TATJANA</au><au>GREMSE, MARION</au><au>KLAES, ANDREA</au><au>RIEMEN, GUDULA</au><au>HELFRICH, JULIANA</au><au>SCHMIDT, HANNS-MARTIN</au><au>SIEBENKOTTEN, GREGOR</au><au>LENZ, DIETMAR</au><au>BROSTERHUS, HELMUT</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>MODULAR TRANSFECTION SYSTEMS BASED ON NUCLEOPROTEIN FILAMENTS</title><date>2011-10-26</date><risdate>2011</risdate><abstract>The present invention relates to a method for transfection of cells using at least one protein capable of forming nucleoprotein filaments, wherein the protein is initially modified with at least one functional component which influences one or more steps of the transfection, the nucleic acid to be transfected is then loaded with the modified protein, whereby the nucleic acid and the protein form a filament-like complex, and this complex is finally added to the cells to be transfected. The invention further relates to a transfection agent consisting of nucleoprotein filaments (NPF), with at least one nucleoprotein filament-forming protein being modified with at least one functional component for the transfection. Furthermore, the present invention relates to the use of the transfection agent according to the invention for producing a drug for gene therapeutic treatment of humans and animals. The present inventions also includes corresponding pharmaceutical preparations, especially for use in gene therapy as well as the use of such transfection agents as component in kits.</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | eng ; fre ; ger |
| recordid | cdi_epo_espacenet_EP1427837B1 |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE |
| title | MODULAR TRANSFECTION SYSTEMS BASED ON NUCLEOPROTEIN FILAMENTS |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T22%3A24%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=ORTMANN,%20BODO&rft.date=2011-10-26&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3EEP1427837B1%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |