METHOD FOR STABLE CHROMOSOMAL MULTI-COPY INTEGRATION OF GENES

METHOD FOR STABLE CHROMOSOMAL MULTI-COPY INTEGRATION OF GENES

The present invention solves the problem of integrating multiple copies of a gene of interest by homologous recombination into well defined positions adjacent to conditionally essential genes in a bacterial host strain chromosome, which already comprises at least one copy of the gene of interest in...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: JORGENSEN, STEEN, TROELS, OLSEN, CARSTEN, ANDERSEN, JENS, TOENNE, RASMUSSEN, MICHAEL, DOLBJERG
Format: Patent
Sprache:eng ; fre ; ger
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The present invention solves the problem of integrating multiple copies of a gene of interest by homologous recombination into well defined positions adjacent to conditionally essential genes in a bacterial host strain chromosome, which already comprises at least one copy of the gene of interest in a different position.