PURIFICATION OF RECOMBINANT HOMOMULTIMERIC VEGF-B

PURIFICATION OF RECOMBINANT HOMOMULTIMERIC VEGF-B

The present invention provides a method for purifying recombinant peptides, polypeptides or proteins away from truncated or other full-length forms of these molecules. In particular the invention contemplates a method of purifying a vascular endothelial growth factor (VEGF) molecule by subjecting a...

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Hauptverfasser: SCOTNEY, PIERRE, D, FABRI, LOUIS, J, NASH, ANDREW, D, MACKENZIE, ANDREW, W, SCROFANI, SERGIO, D., B
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Sprache:eng ; fre ; ger
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CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
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creator SCOTNEY, PIERRE, D
FABRI, LOUIS, J
NASH, ANDREW, D
MACKENZIE, ANDREW, W
SCROFANI, SERGIO, D., B
description The present invention provides a method for purifying recombinant peptides, polypeptides or proteins away from truncated or other full-length forms of these molecules. In particular the invention contemplates a method of purifying a vascular endothelial growth factor (VEGF) molecule by subjecting a biological sample containing the molecule to be purified to affinity chromatography under conditions sufficient for the full length molecules to bind and not the truncated or clipped forms. In the preferred embodiment there are two columns, the first is based on affinity for a poly his tag, the second column based on heparin binding affinity. Particularly preferred VEGF molecules are untagged VEGF-B167, hexa-His-tagged VEGF-B167, hexa-His-tagged VEGF-B186 and hexa-His-tagged VEGF-B10-108.
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subjects CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
title PURIFICATION OF RECOMBINANT HOMOMULTIMERIC VEGF-B
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