METHOD FOR MATURATION OF CONIFER SOMATIC EMBRYOS
According to the invention an embryogenic cell mass is cultured with a culture medium comprising an anti-auxin resulting in an unexpected shift in physiology from proliferation to maturation. Proliferation is reduced so that the formation of new immature embryos ceases. Reduction of proliferation fa...
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description | According to the invention an embryogenic cell mass is cultured with a culture medium comprising an anti-auxin resulting in an unexpected shift in physiology from proliferation to maturation. Proliferation is reduced so that the formation of new immature embryos ceases. Reduction of proliferation facilitates the transition from proliferation to maturation and maturation frequency is increased to a much larger extend than expected. Surprisingly, it has been discovered that the quality of the somatic embryos is not reduced, although the activity of the important endogenous plant growth regulator, auxin, is reduced. As a matter of fact, the overall quality of the mature embryos harvested at the end of maturation is actually increased over the prior art. |
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Proliferation is reduced so that the formation of new immature embryos ceases. Reduction of proliferation facilitates the transition from proliferation to maturation and maturation frequency is increased to a much larger extend than expected. Surprisingly, it has been discovered that the quality of the somatic embryos is not reduced, although the activity of the important endogenous plant growth regulator, auxin, is reduced. As a matter of fact, the overall quality of the mature embryos harvested at the end of maturation is actually increased over the prior art.</description><edition>7</edition><language>eng ; fre ; ger</language><subject>AGRICULTURE ; ANIMAL HUSBANDRY ; FISHING ; FORESTRY ; HUMAN NECESSITIES ; HUNTING ; NEW PLANTS OR PROCESSES FOR OBTAINING THEM ; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES ; TRAPPING</subject><creationdate>2002</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20020703&DB=EPODOC&CC=EP&NR=1217884A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20020703&DB=EPODOC&CC=EP&NR=1217884A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>FIND, JENS, IVER</creatorcontrib><title>METHOD FOR MATURATION OF CONIFER SOMATIC EMBRYOS</title><description>According to the invention an embryogenic cell mass is cultured with a culture medium comprising an anti-auxin resulting in an unexpected shift in physiology from proliferation to maturation. Proliferation is reduced so that the formation of new immature embryos ceases. Reduction of proliferation facilitates the transition from proliferation to maturation and maturation frequency is increased to a much larger extend than expected. Surprisingly, it has been discovered that the quality of the somatic embryos is not reduced, although the activity of the important endogenous plant growth regulator, auxin, is reduced. As a matter of fact, the overall quality of the mature embryos harvested at the end of maturation is actually increased over the prior art.</description><subject>AGRICULTURE</subject><subject>ANIMAL HUSBANDRY</subject><subject>FISHING</subject><subject>FORESTRY</subject><subject>HUMAN NECESSITIES</subject><subject>HUNTING</subject><subject>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</subject><subject>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</subject><subject>TRAPPING</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2002</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZDDwdQ3x8HdRcPMPUvB1DAkNcgzx9PdT8HdTcPb383RzDVII9geKezoruPo6BUX6B_MwsKYl5hSn8kJpbgYFN9cQZw_d1IL8-NTigsTk1LzUknjXAEMjQ3MLCxNHQ2MilAAA53ol9Q</recordid><startdate>20020703</startdate><enddate>20020703</enddate><creator>FIND, JENS, IVER</creator><scope>EVB</scope></search><sort><creationdate>20020703</creationdate><title>METHOD FOR MATURATION OF CONIFER SOMATIC EMBRYOS</title><author>FIND, JENS, IVER</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_EP1217884A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre ; ger</language><creationdate>2002</creationdate><topic>AGRICULTURE</topic><topic>ANIMAL HUSBANDRY</topic><topic>FISHING</topic><topic>FORESTRY</topic><topic>HUMAN NECESSITIES</topic><topic>HUNTING</topic><topic>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</topic><topic>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</topic><topic>TRAPPING</topic><toplevel>online_resources</toplevel><creatorcontrib>FIND, JENS, IVER</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>FIND, JENS, IVER</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>METHOD FOR MATURATION OF CONIFER SOMATIC EMBRYOS</title><date>2002-07-03</date><risdate>2002</risdate><abstract>According to the invention an embryogenic cell mass is cultured with a culture medium comprising an anti-auxin resulting in an unexpected shift in physiology from proliferation to maturation. Proliferation is reduced so that the formation of new immature embryos ceases. Reduction of proliferation facilitates the transition from proliferation to maturation and maturation frequency is increased to a much larger extend than expected. Surprisingly, it has been discovered that the quality of the somatic embryos is not reduced, although the activity of the important endogenous plant growth regulator, auxin, is reduced. As a matter of fact, the overall quality of the mature embryos harvested at the end of maturation is actually increased over the prior art.</abstract><edition>7</edition><oa>free_for_read</oa></addata></record> |
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subjects | AGRICULTURE ANIMAL HUSBANDRY FISHING FORESTRY HUMAN NECESSITIES HUNTING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES TRAPPING |
title | METHOD FOR MATURATION OF CONIFER SOMATIC EMBRYOS |
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