PARTICULATE MATERIAL FOR FLUIDISED BED PURIFICATION OF BIO-MACROMOLECULES SUCH AS PLASMID DNA, CHROMOSOMAL DNA, RNA, VIRAL DNA, BACTERIA AND VIRUSES

The present invention relates to particulate material having a density of at least 2.5 g/ml, where the particles of the particulate material have an average diameter of 5-75 mu m, and the particles of the particulate material are essentially constructed of a polymeric base matrix, e.g. a polysacchar...

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Hauptverfasser: LIHME, ALLAN, OTTO, FOG, SIMON, MARCOS, THEODOSSIOU, IRINI, THOMAS, OWEN, ROBERT, TYRYNIS, OLANDER, MORTEN, AAE, HOBLEY, TIMOTHY, JOHN
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creator LIHME, ALLAN, OTTO, FOG
SIMON, MARCOS
THEODOSSIOU, IRINI
THOMAS, OWEN, ROBERT, TYRYNIS
OLANDER, MORTEN, AAE
HOBLEY, TIMOTHY, JOHN
description The present invention relates to particulate material having a density of at least 2.5 g/ml, where the particles of the particulate material have an average diameter of 5-75 mu m, and the particles of the particulate material are essentially constructed of a polymeric base matrix, e.g. a polysaccharide such as agarose, and a non-porous core material, e.g. steel and titanium, said core material having a density of at least 3.0 g/ml, said polymeric base matrix including pendant groups which are positively charged at pH 4.0 or which are affinity ligands for a bio-molecule. Possible pendant groups include polyethyleneimine (PEI), diethylaminoethyl (DEAE) and quaternary aminoethyl (QAE). The materials are useful in expanded bed or fluidised bed chromatography processes, in particular for purification of bio-macromolecules such as plasmid DNA, chromosomal DNA, RNA, viral DNA, bacteria and viruses.
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language eng ; fre ; ger
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subjects BEER
BIOCHEMISTRY
CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SEPARATION
SPIRITS
THEIR RELEVANT APPARATUS
TRANSPORTING
VINEGAR
WINE
title PARTICULATE MATERIAL FOR FLUIDISED BED PURIFICATION OF BIO-MACROMOLECULES SUCH AS PLASMID DNA, CHROMOSOMAL DNA, RNA, VIRAL DNA, BACTERIA AND VIRUSES
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