Rapid assay for detection of nucleic acid-binding factors

This invention consists of methods for the detection of nucleotide-binding compounds in complex fluid mixtures. The invention consists of fixing a target polynucleotide of known sequence to a non-porous support which comprises or is contained within a container. The challenging sample fluid is then...

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Hauptverfasser: YAO, RAYMOND CHE-FONG, MEIER, TIMOTHY IVAN
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creator YAO, RAYMOND CHE-FONG
MEIER, TIMOTHY IVAN
description This invention consists of methods for the detection of nucleotide-binding compounds in complex fluid mixtures. The invention consists of fixing a target polynucleotide of known sequence to a non-porous support which comprises or is contained within a container. The challenging sample fluid is then introduced in such a way that compounds present in the fluid bind to the target polynucleotide. This binding blocks the hybridization of a second, complementary polynucleotide to which is attached a label comprising a moiety capable of generating a detectable signal. The invention further provides for indirect means of binding the target polynucleotide to the support and of generating the detectable signal.
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language eng ; fre ; ger
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
title Rapid assay for detection of nucleic acid-binding factors
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