ASSAY METHODS AND COMPOSITIONS FOR DETECTING AND EVALUATING THE INTRACELLULAR TRANSDUCTION OF AN EXTRACELLULAR SIGNAL

Techniques basées sur la transcription identifiant les signaux extracellulaires qui modulent l'activité des protéines de surface cellulaire. On identifie les signaux extracellulaires en mesurant la quantité de transcription d'un gène rapporteur dans une cellule recombinée exprimant la prot...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: BRUST, PAUL, HARPOLD, MICHAEL, MILLER
Format: Patent
Sprache:eng ; fre ; ger
Schlagworte:
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue
container_start_page
container_title
container_volume
creator BRUST, PAUL
HARPOLD, MICHAEL, MILLER
description Techniques basées sur la transcription identifiant les signaux extracellulaires qui modulent l'activité des protéines de surface cellulaire. On identifie les signaux extracellulaires en mesurant la quantité de transcription d'un gène rapporteur dans une cellule recombinée exprimant la protéine de surface cellulaire et contenant de l'ADN codant le gène rapporteur dont la transcription est régulée, directement ou indirectement, par ladite protéine de surface cellulaire. Les techniques servent de moyen d'identification des composés pharmaceutiques potentiels qui peuvent s'utiliser pour traiter des maladies du fait de leurs effets agonistes ou antagonistes sur la protéine de surface cellulaire. On a également prévu des cellules recombinées exprimant des récepteurs de surface cellulaire et contenant des structures de gène rapporteur qui comprennent des éléments régulateurs de transcription sensibles à l'activité des récepteurs de surface cellulaire. Transcription based assays that identify extracellular signals that modulate the activity of cell surface proteins are provided. Extracellular signals are identified by measuring the amount of transcription of a reporter gene in a recombinant cell that expresses the cell surface protein and contains DNA encoding the reporter gene under the transcriptional control of a promoter that is regulated, directly or indirectly, by he cell surface protein. The assays provide a means for identifying potential pharmaceutical compounds that can be used to treat disease by virtue of their agonistic or antagonistic effects on the cell surface protein. Recombinant cells that express cell surface receptors and that contain reporter gene constructs that include transcriptional regulatory elements that are responsive to the activity of the cell surface receptors are also provided.
format Patent
fullrecord <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_EP0542830A1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>EP0542830A1</sourcerecordid><originalsourceid>FETCH-epo_espacenet_EP0542830A13</originalsourceid><addsrcrecordid>eNqNi7EKwkAQRNNYiPoP-wNCNAq2y90mObjcheyeaBWCnJVoIPr_xmBhaTUzzHvz5IXMeIaKpPSaAZ0G5avasxHjHUPuG9AkpMS4YrrpiDbgNKUkME4aVGRtsNjA2B3roD4y-HwUgE6_AJvCoV0ms2t3G-Lqm4sEchJVrmP_aOPQd5d4j8-W6nS_2x6yFDfZH8gbpvc5Cw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>ASSAY METHODS AND COMPOSITIONS FOR DETECTING AND EVALUATING THE INTRACELLULAR TRANSDUCTION OF AN EXTRACELLULAR SIGNAL</title><source>esp@cenet</source><creator>BRUST, PAUL ; HARPOLD, MICHAEL, MILLER</creator><creatorcontrib>BRUST, PAUL ; HARPOLD, MICHAEL, MILLER</creatorcontrib><description>Techniques basées sur la transcription identifiant les signaux extracellulaires qui modulent l'activité des protéines de surface cellulaire. On identifie les signaux extracellulaires en mesurant la quantité de transcription d'un gène rapporteur dans une cellule recombinée exprimant la protéine de surface cellulaire et contenant de l'ADN codant le gène rapporteur dont la transcription est régulée, directement ou indirectement, par ladite protéine de surface cellulaire. Les techniques servent de moyen d'identification des composés pharmaceutiques potentiels qui peuvent s'utiliser pour traiter des maladies du fait de leurs effets agonistes ou antagonistes sur la protéine de surface cellulaire. On a également prévu des cellules recombinées exprimant des récepteurs de surface cellulaire et contenant des structures de gène rapporteur qui comprennent des éléments régulateurs de transcription sensibles à l'activité des récepteurs de surface cellulaire. Transcription based assays that identify extracellular signals that modulate the activity of cell surface proteins are provided. Extracellular signals are identified by measuring the amount of transcription of a reporter gene in a recombinant cell that expresses the cell surface protein and contains DNA encoding the reporter gene under the transcriptional control of a promoter that is regulated, directly or indirectly, by he cell surface protein. The assays provide a means for identifying potential pharmaceutical compounds that can be used to treat disease by virtue of their agonistic or antagonistic effects on the cell surface protein. Recombinant cells that express cell surface receptors and that contain reporter gene constructs that include transcriptional regulatory elements that are responsive to the activity of the cell surface receptors are also provided.</description><edition>5</edition><language>eng ; fre ; ger</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PHYSICS ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TESTING ; VINEGAR ; WINE</subject><creationdate>1993</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=19930526&amp;DB=EPODOC&amp;CC=EP&amp;NR=0542830A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25543,76293</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=19930526&amp;DB=EPODOC&amp;CC=EP&amp;NR=0542830A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>BRUST, PAUL</creatorcontrib><creatorcontrib>HARPOLD, MICHAEL, MILLER</creatorcontrib><title>ASSAY METHODS AND COMPOSITIONS FOR DETECTING AND EVALUATING THE INTRACELLULAR TRANSDUCTION OF AN EXTRACELLULAR SIGNAL</title><description>Techniques basées sur la transcription identifiant les signaux extracellulaires qui modulent l'activité des protéines de surface cellulaire. On identifie les signaux extracellulaires en mesurant la quantité de transcription d'un gène rapporteur dans une cellule recombinée exprimant la protéine de surface cellulaire et contenant de l'ADN codant le gène rapporteur dont la transcription est régulée, directement ou indirectement, par ladite protéine de surface cellulaire. Les techniques servent de moyen d'identification des composés pharmaceutiques potentiels qui peuvent s'utiliser pour traiter des maladies du fait de leurs effets agonistes ou antagonistes sur la protéine de surface cellulaire. On a également prévu des cellules recombinées exprimant des récepteurs de surface cellulaire et contenant des structures de gène rapporteur qui comprennent des éléments régulateurs de transcription sensibles à l'activité des récepteurs de surface cellulaire. Transcription based assays that identify extracellular signals that modulate the activity of cell surface proteins are provided. Extracellular signals are identified by measuring the amount of transcription of a reporter gene in a recombinant cell that expresses the cell surface protein and contains DNA encoding the reporter gene under the transcriptional control of a promoter that is regulated, directly or indirectly, by he cell surface protein. The assays provide a means for identifying potential pharmaceutical compounds that can be used to treat disease by virtue of their agonistic or antagonistic effects on the cell surface protein. Recombinant cells that express cell surface receptors and that contain reporter gene constructs that include transcriptional regulatory elements that are responsive to the activity of the cell surface receptors are also provided.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PHYSICS</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TESTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>1993</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNi7EKwkAQRNNYiPoP-wNCNAq2y90mObjcheyeaBWCnJVoIPr_xmBhaTUzzHvz5IXMeIaKpPSaAZ0G5avasxHjHUPuG9AkpMS4YrrpiDbgNKUkME4aVGRtsNjA2B3roD4y-HwUgE6_AJvCoV0ms2t3G-Lqm4sEchJVrmP_aOPQd5d4j8-W6nS_2x6yFDfZH8gbpvc5Cw</recordid><startdate>19930526</startdate><enddate>19930526</enddate><creator>BRUST, PAUL</creator><creator>HARPOLD, MICHAEL, MILLER</creator><scope>EVB</scope></search><sort><creationdate>19930526</creationdate><title>ASSAY METHODS AND COMPOSITIONS FOR DETECTING AND EVALUATING THE INTRACELLULAR TRANSDUCTION OF AN EXTRACELLULAR SIGNAL</title><author>BRUST, PAUL ; HARPOLD, MICHAEL, MILLER</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_EP0542830A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre ; ger</language><creationdate>1993</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PHYSICS</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>TESTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>BRUST, PAUL</creatorcontrib><creatorcontrib>HARPOLD, MICHAEL, MILLER</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>BRUST, PAUL</au><au>HARPOLD, MICHAEL, MILLER</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>ASSAY METHODS AND COMPOSITIONS FOR DETECTING AND EVALUATING THE INTRACELLULAR TRANSDUCTION OF AN EXTRACELLULAR SIGNAL</title><date>1993-05-26</date><risdate>1993</risdate><abstract>Techniques basées sur la transcription identifiant les signaux extracellulaires qui modulent l'activité des protéines de surface cellulaire. On identifie les signaux extracellulaires en mesurant la quantité de transcription d'un gène rapporteur dans une cellule recombinée exprimant la protéine de surface cellulaire et contenant de l'ADN codant le gène rapporteur dont la transcription est régulée, directement ou indirectement, par ladite protéine de surface cellulaire. Les techniques servent de moyen d'identification des composés pharmaceutiques potentiels qui peuvent s'utiliser pour traiter des maladies du fait de leurs effets agonistes ou antagonistes sur la protéine de surface cellulaire. On a également prévu des cellules recombinées exprimant des récepteurs de surface cellulaire et contenant des structures de gène rapporteur qui comprennent des éléments régulateurs de transcription sensibles à l'activité des récepteurs de surface cellulaire. Transcription based assays that identify extracellular signals that modulate the activity of cell surface proteins are provided. Extracellular signals are identified by measuring the amount of transcription of a reporter gene in a recombinant cell that expresses the cell surface protein and contains DNA encoding the reporter gene under the transcriptional control of a promoter that is regulated, directly or indirectly, by he cell surface protein. The assays provide a means for identifying potential pharmaceutical compounds that can be used to treat disease by virtue of their agonistic or antagonistic effects on the cell surface protein. Recombinant cells that express cell surface receptors and that contain reporter gene constructs that include transcriptional regulatory elements that are responsive to the activity of the cell surface receptors are also provided.</abstract><edition>5</edition><oa>free_for_read</oa></addata></record>
fulltext fulltext_linktorsrc
identifier
ispartof
issn
language eng ; fre ; ger
recordid cdi_epo_espacenet_EP0542830A1
source esp@cenet
subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
COMPOSITIONS THEREOF
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
CULTURE MEDIA
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PHYSICS
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
TESTING
VINEGAR
WINE
title ASSAY METHODS AND COMPOSITIONS FOR DETECTING AND EVALUATING THE INTRACELLULAR TRANSDUCTION OF AN EXTRACELLULAR SIGNAL
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-25T04%3A12%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=BRUST,%20PAUL&rft.date=1993-05-26&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3EEP0542830A1%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true