METHOD FOR SEQUENCING OF DEOXYRIBONUCLEIC ACID

The method comprises subjecting the deoxyribonucleic acid in a concentration of 10 to 2 x 10 mol per mu l, in the case of double-stranded deoxynucleic acid which is not in the supercoiled form preferably in a concentration of 10 to 3 x 10 mol per mu l, a) to a heat-denaturation step b) to an anneali...

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Bibliographische Detailangaben
1. Verfasser:	WOZNY, MANFRED
Format:	Patent
Sprache:	eng ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The method comprises subjecting the deoxyribonucleic acid in a concentration of 10 to 2 x 10 mol per mu l, in the case of double-stranded deoxynucleic acid which is not in the supercoiled form preferably in a concentration of 10 to 3 x 10 mol per mu l, a) to a heat-denaturation step b) to an annealing step and c) to a nucleic acid synthesis step with partial chain termination, repeating steps a) to c) 10 to 60 times and subsequently fractionating the resulting deoxynucleic acid fragment mixture according to length, and determining the sequence from the fragment spectrum. o