Method for the determination of HIV antibodies
The method operates on the principle of the heterogenous immunoassay and is characterised in that the sample is incubated simultaneously or sequentially in a reaction vessel to whose internal surface streptavidin is bound in an amount such that 0.1 - 2.5 mu g are present per ml of reaction volume a...
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| creator | KLEIN, CHRISTIAN BAYER, HUBERT |
| description | The method operates on the principle of the heterogenous immunoassay and is characterised in that the sample is incubated simultaneously or sequentially in a reaction vessel to whose internal surface streptavidin is bound in an amount such that 0.1 - 2.5 mu g are present per ml of reaction volume
a) with at least one biotinylated polypeptide which consists of a maximum of 50 amino-acid residues and contains the amino acid sequence CAFRQVC and/or CSGKLIC, where in each case the two cysteines (C) of the two amino-acid sequences are cyclised to cystine bridges, and the polypeptide is monomeric,
b) and with a labelled receptor which is directed against HIV antibodies, the liquid and solid phases are separated and the label in one of the two phases is determined as a measure of the HIV antibody. |
| format | Patent |
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a) with at least one biotinylated polypeptide which consists of a maximum of 50 amino-acid residues and contains the amino acid sequence CAFRQVC and/or CSGKLIC, where in each case the two cysteines (C) of the two amino-acid sequences are cyclised to cystine bridges, and the polypeptide is monomeric,
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a) with at least one biotinylated polypeptide which consists of a maximum of 50 amino-acid residues and contains the amino acid sequence CAFRQVC and/or CSGKLIC, where in each case the two cysteines (C) of the two amino-acid sequences are cyclised to cystine bridges, and the polypeptide is monomeric,
b) and with a labelled receptor which is directed against HIV antibodies, the liquid and solid phases are separated and the label in one of the two phases is determined as a measure of the HIV antibody.</description><subject>CHEMISTRY</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>METALLURGY</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PHYSICS</subject><subject>TESTING</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>1995</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZNDzTS3JyE9RSMsvUijJSFVISS1JLcrNzEssyczPU8hPU_DwDFNIzCvJTMpPyUwt5mFgTUvMKU7lhdLcDApuriHOHrqpBfnxqcUFicmpeakl8a4BBsbmlpYmlk6GxkQoAQB3CinL</recordid><startdate>19950329</startdate><enddate>19950329</enddate><creator>KLEIN, CHRISTIAN</creator><creator>BAYER, HUBERT</creator><scope>EVB</scope></search><sort><creationdate>19950329</creationdate><title>Method for the determination of HIV antibodies</title><author>KLEIN, CHRISTIAN ; BAYER, HUBERT</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_EP0379949B13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre ; ger</language><creationdate>1995</creationdate><topic>CHEMISTRY</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>METALLURGY</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PHYSICS</topic><topic>TESTING</topic><toplevel>online_resources</toplevel><creatorcontrib>KLEIN, CHRISTIAN</creatorcontrib><creatorcontrib>BAYER, HUBERT</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>KLEIN, CHRISTIAN</au><au>BAYER, HUBERT</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method for the determination of HIV antibodies</title><date>1995-03-29</date><risdate>1995</risdate><abstract>The method operates on the principle of the heterogenous immunoassay and is characterised in that the sample is incubated simultaneously or sequentially in a reaction vessel to whose internal surface streptavidin is bound in an amount such that 0.1 - 2.5 mu g are present per ml of reaction volume
a) with at least one biotinylated polypeptide which consists of a maximum of 50 amino-acid residues and contains the amino acid sequence CAFRQVC and/or CSGKLIC, where in each case the two cysteines (C) of the two amino-acid sequences are cyclised to cystine bridges, and the polypeptide is monomeric,
b) and with a labelled receptor which is directed against HIV antibodies, the liquid and solid phases are separated and the label in one of the two phases is determined as a measure of the HIV antibody.</abstract><edition>5</edition><oa>free_for_read</oa></addata></record> |
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| language | eng ; fre ; ger |
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| subjects | CHEMISTRY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY ORGANIC CHEMISTRY PEPTIDES PHYSICS TESTING |
| title | Method for the determination of HIV antibodies |
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