Diagnostic test for virus desease and method for the cryopreservation of cells used in these tests
Diagnostic test for viral disease and process for freezing cells which can be used for this test. The present invention relates to a diagnostic test for viral disease comprising the use of cells making it possible to amplify the virus to be identified from samples and thus making its detection possi...
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| creator | PIRSON, PHILIPPE JEAN ACHILLE LOUIS |
| description | Diagnostic test for viral disease and process for freezing cells which can be used for this test. The present invention relates to a diagnostic test for viral disease comprising the use of cells making it possible to amplify the virus to be identified from samples and thus making its detection possible, characterised in that use is made of frozen cells which are thawed in order to perform the test. The present invention also relates to a process for freezing cells in a support container consisting especially of multiwell plates or tubes permitting the preservation of cells and their use after thawing in a diagnostic test according to the invention, characterised in that 1) a cryopreservative is employed, chosen so that the number of viable and functional cells after thawing is optimised and especially higher than 80% and, 2) the number of cells to be frozen which are introduced into the support container is chosen so that 24 hours after thawing the cultured cells form a complete cellular monolayer. |
| format | Patent |
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The present invention relates to a diagnostic test for viral disease comprising the use of cells making it possible to amplify the virus to be identified from samples and thus making its detection possible, characterised in that use is made of frozen cells which are thawed in order to perform the test. The present invention also relates to a process for freezing cells in a support container consisting especially of multiwell plates or tubes permitting the preservation of cells and their use after thawing in a diagnostic test according to the invention, characterised in that 1) a cryopreservative is employed, chosen so that the number of viable and functional cells after thawing is optimised and especially higher than 80% and, 2) the number of cells to be frozen which are introduced into the support container is chosen so that 24 hours after thawing the cultured cells form a complete cellular monolayer.</description><language>eng ; fre ; ger</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PHYSICS ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TESTING ; VINEGAR ; WINE</subject><creationdate>1989</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19891123&DB=EPODOC&CC=EP&NR=0343080A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19891123&DB=EPODOC&CC=EP&NR=0343080A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>PIRSON, PHILIPPE JEAN ACHILLE LOUIS</creatorcontrib><title>Diagnostic test for virus desease and method for the cryopreservation of cells used in these tests</title><description>Diagnostic test for viral disease and process for freezing cells which can be used for this test. The present invention relates to a diagnostic test for viral disease comprising the use of cells making it possible to amplify the virus to be identified from samples and thus making its detection possible, characterised in that use is made of frozen cells which are thawed in order to perform the test. The present invention also relates to a process for freezing cells in a support container consisting especially of multiwell plates or tubes permitting the preservation of cells and their use after thawing in a diagnostic test according to the invention, characterised in that 1) a cryopreservative is employed, chosen so that the number of viable and functional cells after thawing is optimised and especially higher than 80% and, 2) the number of cells to be frozen which are introduced into the support container is chosen so that 24 hours after thawing the cultured cells form a complete cellular monolayer.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PHYSICS</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TESTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>1989</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjDEOwjAQBNNQIOAP9wEkI1PQIgiipKCPjL0hlowv8l0i8XsSxAOotpiZXVaPc3TPzKLRk0KUWi40xjIIBQicgFwO9IJ2HL5QO5Avb-7LxMvoNHImbskjJaFBECjm2ZrS-VHW1aJ1SbD57aqiS30_XbfouYH0ziNDm_pm7N6agznu7B_KB-HXPas</recordid><startdate>19891123</startdate><enddate>19891123</enddate><creator>PIRSON, PHILIPPE JEAN ACHILLE LOUIS</creator><scope>EVB</scope></search><sort><creationdate>19891123</creationdate><title>Diagnostic test for virus desease and method for the cryopreservation of cells used in these tests</title><author>PIRSON, PHILIPPE JEAN ACHILLE LOUIS</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_EP0343080A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre ; ger</language><creationdate>1989</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PHYSICS</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>TESTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>PIRSON, PHILIPPE JEAN ACHILLE LOUIS</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>PIRSON, PHILIPPE JEAN ACHILLE LOUIS</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Diagnostic test for virus desease and method for the cryopreservation of cells used in these tests</title><date>1989-11-23</date><risdate>1989</risdate><abstract>Diagnostic test for viral disease and process for freezing cells which can be used for this test. The present invention relates to a diagnostic test for viral disease comprising the use of cells making it possible to amplify the virus to be identified from samples and thus making its detection possible, characterised in that use is made of frozen cells which are thawed in order to perform the test. The present invention also relates to a process for freezing cells in a support container consisting especially of multiwell plates or tubes permitting the preservation of cells and their use after thawing in a diagnostic test according to the invention, characterised in that 1) a cryopreservative is employed, chosen so that the number of viable and functional cells after thawing is optimised and especially higher than 80% and, 2) the number of cells to be frozen which are introduced into the support container is chosen so that 24 hours after thawing the cultured cells form a complete cellular monolayer.</abstract><oa>free_for_read</oa></addata></record> |
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| language | eng ; fre ; ger |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE |
| title | Diagnostic test for virus desease and method for the cryopreservation of cells used in these tests |
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