Immunoassay utilizing biotin bridge with universal solid phase
An immunoassay utilizing a biotin bridge is performed with a solid phase with anti-biotin immobilized on its surface. In accordance with the assay, the solid phase is incubated with a biotinylated binding species, preferably biotinylated antibody, specific for the ligand to be detected. This is then...
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| creator | MURTHY, SHASHIDHARA H.M BREGLIO, SUSAN E PHILLIPS, VINCENT E |
| description | An immunoassay utilizing a biotin bridge is performed with a solid phase with anti-biotin immobilized on its surface. In accordance with the assay, the solid phase is incubated with a biotinylated binding species, preferably biotinylated antibody, specific for the ligand to be detected. This is then reacted with the test sample, followed by a labeled binding member, either in a sandwich-type or competitive-type binding assay. The label is then detected in accordance with conventional detection techniques. Use of the solid phase with only a biotin-specific antibody attached thereto expands the utility and versatility of a single solid phase for use in the detection of an unlimited variety of ligands. |
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In accordance with the assay, the solid phase is incubated with a biotinylated binding species, preferably biotinylated antibody, specific for the ligand to be detected. This is then reacted with the test sample, followed by a labeled binding member, either in a sandwich-type or competitive-type binding assay. The label is then detected in accordance with conventional detection techniques. Use of the solid phase with only a biotin-specific antibody attached thereto expands the utility and versatility of a single solid phase for use in the detection of an unlimited variety of ligands.</abstract><edition>4</edition><oa>free_for_read</oa></addata></record> |
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| subjects | HUMAN NECESSITIES HYGIENE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEDICAL OR VETERINARY SCIENCE PHYSICS PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES TESTING |
| title | Immunoassay utilizing biotin bridge with universal solid phase |
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