A functional assay for T-Lymphocytes

The presence of activated or pre-sensitized T-lymphocytes in whole blood, or in a mixture of isolated leukocytes, is detected by means of forming autologous E-rosettes and mixed leukocyte-erythrocyte aggregates which are stable at 37 DEG C. More specifically, by means of stimulating the cells using...

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Bibliographische Detailangaben
Hauptverfasser:	KUCHLER, MARTHA, LIAO, MEI-JUNE, KHAVKIN, THEODOR
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The presence of activated or pre-sensitized T-lymphocytes in whole blood, or in a mixture of isolated leukocytes, is detected by means of forming autologous E-rosettes and mixed leukocyte-erythrocyte aggregates which are stable at 37 DEG C. More specifically, by means of stimulating the cells using either a non-erythroagglutinating mitogen or an antigen, and removing sialic acid with, for example, neuraminidase, stable E-rosettes and mixed leukocyte-erythrocyte aggregates may be formed and counted. Also provided are kits for use in assaying for the presence of mitogen-activated or antigen pre-sensitized T-lymphocytes, without using sheep red blood cells, immunostaining or radioactive isotopes.