Apparatus and method for culturing cells, removing waste and concentrating product
An apparatus and method for culturing cells includes a waste removal and product concentrating loop in which waste is removed from the medium and product produced by the cells is concentrated for harvesting. The apparatus includes a reservoir (12) for retaining a supply of medium. The reservoir is i...
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creator | HIRSCHEL, MARK D GRUENBERG, MICHAEL L HARM, WILLIAM H |
description | An apparatus and method for culturing cells includes a waste removal and product concentrating loop in which waste is removed from the medium and product produced by the cells is concentrated for harvesting. The apparatus includes a reservoir (12) for retaining a supply of medium. The reservoir is in fluid communication with a cell culturing loop (14) wherein medium flows from the reservoir to the cells and back to the reservoir. The medium waste removal and concentrating loop (16) removes waste components produced by the cells and is in fluid communication with the reservoir such that medium is transferred from the reservoir to the waste removal and concentrating loop and back to the reservoir. The waste removal and concentrating loop includes a device having a semipermeable membrane (50). The medium flows along one side of the membrane for selective transfer of the waste components through the membrane. A mechanism for selectively producing a back pressure (52) in the medium is included downstream of the membrane and aids in transfer of waste components through the membrane to remove the waste components from the medium. |
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The apparatus includes a reservoir (12) for retaining a supply of medium. The reservoir is in fluid communication with a cell culturing loop (14) wherein medium flows from the reservoir to the cells and back to the reservoir. The medium waste removal and concentrating loop (16) removes waste components produced by the cells and is in fluid communication with the reservoir such that medium is transferred from the reservoir to the waste removal and concentrating loop and back to the reservoir. The waste removal and concentrating loop includes a device having a semipermeable membrane (50). The medium flows along one side of the membrane for selective transfer of the waste components through the membrane. A mechanism for selectively producing a back pressure (52) in the medium is included downstream of the membrane and aids in transfer of waste components through the membrane to remove the waste components from the medium.</description><edition>4</edition><language>eng ; fre ; ger</language><subject>APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY ; BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>1987</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19870610&DB=EPODOC&CC=EP&NR=0224734A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25563,76318</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19870610&DB=EPODOC&CC=EP&NR=0224734A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>HIRSCHEL, MARK D</creatorcontrib><creatorcontrib>GRUENBERG, MICHAEL L</creatorcontrib><creatorcontrib>HARM, WILLIAM H</creatorcontrib><title>Apparatus and method for culturing cells, removing waste and concentrating product</title><description>An apparatus and method for culturing cells includes a waste removal and product concentrating loop in which waste is removed from the medium and product produced by the cells is concentrated for harvesting. The apparatus includes a reservoir (12) for retaining a supply of medium. The reservoir is in fluid communication with a cell culturing loop (14) wherein medium flows from the reservoir to the cells and back to the reservoir. The medium waste removal and concentrating loop (16) removes waste components produced by the cells and is in fluid communication with the reservoir such that medium is transferred from the reservoir to the waste removal and concentrating loop and back to the reservoir. The waste removal and concentrating loop includes a device having a semipermeable membrane (50). The medium flows along one side of the membrane for selective transfer of the waste components through the membrane. 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The apparatus includes a reservoir (12) for retaining a supply of medium. The reservoir is in fluid communication with a cell culturing loop (14) wherein medium flows from the reservoir to the cells and back to the reservoir. The medium waste removal and concentrating loop (16) removes waste components produced by the cells and is in fluid communication with the reservoir such that medium is transferred from the reservoir to the waste removal and concentrating loop and back to the reservoir. The waste removal and concentrating loop includes a device having a semipermeable membrane (50). The medium flows along one side of the membrane for selective transfer of the waste components through the membrane. A mechanism for selectively producing a back pressure (52) in the medium is included downstream of the membrane and aids in transfer of waste components through the membrane to remove the waste components from the medium.</abstract><edition>4</edition><oa>free_for_read</oa></addata></record> |
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subjects | APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
title | Apparatus and method for culturing cells, removing waste and concentrating product |
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