Ultrahigh copy number streptomycetes plasmids

The present invention discloses an ∼1.4 kb BclI-Sau3A restriction fragment containing the minimal replicon of the Streptomycescoelicolor plasmid SCP2*. This minimal replicon was deduced from plasmid derivatives constructed by invitro deletions and has been identified as the smallest, self-replicati...

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Hauptverfasser:	HERSHBERGER, CHARLES LEE, LARSON, JEFFREY LYNN, REYNOLDS, PATRICIA ANN
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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creator	HERSHBERGER, CHARLES LEE LARSON, JEFFREY LYNN REYNOLDS, PATRICIA ANN
description	The present invention discloses an ∼1.4 kb BclI-Sau3A restriction fragment containing the minimal replicon of the Streptomycescoelicolor plasmid SCP2. This minimal replicon was deduced from plasmid derivatives constructed by invitro deletions and has been identified as the smallest, self-replicating segment of the SCP2 plasmid. The minimal replicon specifies an ultrahigh level of plasmid DNA (more than 1000 fold increase from the basic replicon) when vectors containing this sequence are transformed into Streptomyceslividans. This replicon can be used to construct smaller, more efficient recombinant DNA shuttle vectors to clone DNA into streptomycetes.
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This minimal replicon was deduced from plasmid derivatives constructed by invitro deletions and has been identified as the smallest, self-replicating segment of the SCP2 plasmid. The minimal replicon specifies an ultrahigh level of plasmid DNA (more than 1000 fold increase from the basic replicon) when vectors containing this sequence are transformed into Streptomyceslividans. 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This minimal replicon was deduced from plasmid derivatives constructed by invitro deletions and has been identified as the smallest, self-replicating segment of the SCP2 plasmid. The minimal replicon specifies an ultrahigh level of plasmid DNA (more than 1000 fold increase from the basic replicon) when vectors containing this sequence are transformed into Streptomyceslividans. 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This minimal replicon was deduced from plasmid derivatives constructed by invitro deletions and has been identified as the smallest, self-replicating segment of the SCP2 plasmid. The minimal replicon specifies an ultrahigh level of plasmid DNA (more than 1000 fold increase from the basic replicon) when vectors containing this sequence are transformed into Streptomyceslividans. This replicon can be used to construct smaller, more efficient recombinant DNA shuttle vectors to clone DNA into streptomycetes.</abstract><edition>4</edition><oa>free_for_read</oa></addata></record>
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subjects	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
title	Ultrahigh copy number streptomycetes plasmids
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