Glutamaatdehüdrogenaasi, ß-N-atsetüülheksoosaminidaasi ja y-aktiini geenide promootorid ning nende kasutamine niitseente ekspressiooni-, sekretsiooni- ja antisenss-süsteemides

The invention relates to promoters of the genes glutamate dehydrogenase, beta-acetylhexosaminidase and gamma-actin and their use in systems of expression, secretion and anti-sense of filamentary fungi. The invention also relates to the use of the promoters of the genes which code: (I) glutamate dehy...

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Hauptverfasser: FRANCISCO SALTO MALDONADO, BRUNO DIEZ GARCIA, MIGUEL ANGEL MORENO VALLE, ALFONSO J. COLLADOS DE LA VIEJA, JOSE LUIS BARREDO FUENTE, MARTA RODRIGUEZ SAIZ
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creator FRANCISCO SALTO MALDONADO
BRUNO DIEZ GARCIA
MIGUEL ANGEL MORENO VALLE
ALFONSO J. COLLADOS DE LA VIEJA
JOSE LUIS BARREDO FUENTE
MARTA RODRIGUEZ SAIZ
description The invention relates to promoters of the genes glutamate dehydrogenase, beta-acetylhexosaminidase and gamma-actin and their use in systems of expression, secretion and anti-sense of filamentary fungi. The invention also relates to the use of the promoters of the genes which code: (I) glutamate dehydrogenase NADP depending (EC.1.4.1.4) of Penicillium chrysogenum, (II) gamma-N-actylhexosaminidase (EC.3.2.1.52) of Penicillium chrysogenum and (III) gamma-actin of Penicillium chrysogenum and Acrimonium chrysogenum, which can be used for the construction of potent vectors of expression and secretion useful both for P. chrysogenum and for A. chrysogenum and related species. These promoters can also be used for blocking the genic expression through anti-sense construction. Under the control of the above mentioned promoters, it is possible to conduct the expression of other genes in filamentary fungi, thereby increasing the production of antibiotics and/or proteins inherent to the same.
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title Glutamaatdehüdrogenaasi, ß-N-atsetüülheksoosaminidaasi ja y-aktiini geenide promootorid ning nende kasutamine niitseente ekspressiooni-, sekretsiooni- ja antisenss-süsteemides
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