Fremgangsmåder og sammensætninger til DNA-profilering

Embodiments disclosed herein provide methods for constructing a DNA profile comprising: providing a nucleic acid sample, amplifying the nucleic acid sample with a plurality of primers that specifically hybridize to at least one target sequence comprising a SNP and at least one target sequence compri...

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Hauptverfasser: STEPHENS, Kathryn M, SHEN, Min-jui Richard, JAGER, Anne, HAN, Yonmee, AMINI, Sasan, SILVA, David, DAVIS, Carey, STEEMERS, Frank, WALICHIEWICZ, Paulina, HOLT, Cydne
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creator STEPHENS, Kathryn M
SHEN, Min-jui Richard
JAGER, Anne
HAN, Yonmee
AMINI, Sasan
SILVA, David
DAVIS, Carey
STEEMERS, Frank
WALICHIEWICZ, Paulina
HOLT, Cydne
description Embodiments disclosed herein provide methods for constructing a DNA profile comprising: providing a nucleic acid sample, amplifying the nucleic acid sample with a plurality of primers that specifically hybridize to at least one target sequence comprising a SNP and at least one target sequence comprising a tandem repeat, and determining the genotypes of the at least one SNP and at least one tandem repeat in the amplification products, thereby constructing the DNA profile of the nucleic acid sample. Embodiments disclosed herein further provide a plurality of primers that specifically hybridize to at least one short target sequence and at least one long target sequence in a nucleic acid sample, wherein amplifying the nucleic acid sample using the plurality of primers in a single reaction results in a short amplification product and a long amplification product, wherein each of the plurality of primers comprises one or more tag sequences.
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
title Fremgangsmåder og sammensætninger til DNA-profilering
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