Fremgangsmåde til AFLP-baseret påvisning af polymorfisme med høj omsætning

The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the a...

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Hauptverfasser: VAN EIJK, MICHAEL, JOSEPHUS, THERESIA, VAN SCHRIEK, MARCO, GERARDUS, MARIA, SOERENSEN, ANKER, PREBEN
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creator VAN EIJK, MICHAEL, JOSEPHUS, THERESIA
VAN SCHRIEK, MARCO, GERARDUS, MARIA
SOERENSEN, ANKER, PREBEN
description The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
title Fremgangsmåde til AFLP-baseret påvisning af polymorfisme med høj omsætning
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